Home Cytoskeletal Signaling PKC activator - S1P Receptor activator PMA (Phorbol 12-myristate 13-acetate) For research use only.

PMA (Phorbol 12-myristate 13-acetate)

Synonyms: 12-O-Tetradecanoylphorbol-13-acetate,TPA, Phorbol myristate acetate

PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).PMA has strong excitability on the skin and mucous membrane. Special protection is required when using PMA. Wear gloves and mask to avoid direct contact in any way.

PMA (Phorbol 12-myristate 13-acetate) Chemical Structure

PMA (Phorbol 12-myristate 13-acetate) Chemical Structure

CAS No. 16561-29-8

Purity & Quality Control

Products often used together with PMA (Phorbol 12-myristate 13-acetate)

Tangeretin

Tangeretin significantly inhibits Phorbol 12-myristate 13-acetate (PMA) stimulated epidermal hyperplasia and intra-epidermal neutrophilic abscesses in mice.

Chang SN, et al. Int J Mol Sci. 2020 Dec 4;21(23):9261.

Curcumin

Curcumin reduces the Phorbol 12-myristate 13-acetate (PMA)-induced elevation of uric acid in NIH3T3 cells.

Lin JK, et al. Carcinogenesis. 1994 Aug;15(8):1717-21.

Forskolin (Colforsin)

Phorbol 12-myristate 13-acetate (PMA) and Forskolin are strongly synergistic in altering endothelial cell morphology.

Antonov AS, et al. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9704-8.

PMA (Phorbol 12-myristate 13-acetate) Related Products

Signaling Pathway

PKC Signaling Pathways

PKC Signaling Pathway

Biological Activity

Description PMA (Phorbol 12-myristate 13-acetate), a potent activator of PKC, is active at nanomolar concentrations. Phorbol 12-myristate 13-acetate (PMA) induces sphingosine-1-phosphate (S1P).PMA has strong excitability on the skin and mucous membrane. Special protection is required when using PMA. Wear gloves and mask to avoid direct contact in any way.
In vitro
In vitro

In endothelial cells, PMA increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.[1]
Cell Research Cell lines Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs)
Concentrations 20 ng/mL
Incubation Time 14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein
Method

After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (20ng/mL), and washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.
Experimental Result Images Methods Biomarkers Images PMID
Western blot pERK / ERK1 / pp38 / p38 p-GSK3β / GSK3β / Lamin B2 22977714
Immunofluorescence cTnT / β-MHC / CD90 27405982
In Vivo
In vivo

In the zebrafish model, PMA initially enhances endothelial cell migration, subsequently activates the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.[1]
Animal Research Animal Models Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio)
Dosages 20 ng/mL
Administration --
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06327152 Recruiting
Apnea of Prematurity
University of California Irvine
 February 22 2024 Phase 2
NCT05908227 Recruiting
Respiratory Distress Syndrome
Insel Gruppe AG University Hospital Bern
 July 1 2023 Not Applicable
NCT06318585 Recruiting
Amyotrophic Lateral Sclerosis|Motor Neuron Disease
Istituto Auxologico Italiano
 April 3 2023 --
NCT06087666 Recruiting
Preterm
Instituto de Investigación Hospital Universitario La Paz
 February 1 2023 Not Applicable

Chemical Information & Solubility

Molecular Weight 616.83 Formula

C36H56O8
CAS No. 16561-29-8 SDF --
Smiles CCCCCCCCCCCCCC(=O)OC1C(C2(C(C=C(CC3(C2C=C(C3=O)C)O)CO)C4C1(C4(C)C)OC(=O)C)O)C
Storage (From the date of receipt) 3 years -20°C(in the dark) powder

In vitro
Batch:

DMSO : 100 mg/mL ( (162.11 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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