Home Cytoskeletal Signaling Dynamin inhibitor P110 For research use only.

P110

P110 is a dynamin-related protein 1 (Drp1)-specific inhibitor that binds directly to Drp1.

P110 Chemical Structure

P110 Chemical Structure

CAS No. Unknown

Purity & Quality Control

P110 Related Products

Biological Activity

Description P110 is a dynamin-related protein 1 (Drp1)-specific inhibitor that binds directly to Drp1.
Targets
Drp1 [1]
In vitro
In vitro

P110 is a Drp1-specific inhibitor that binds directly to Drp1. In some cases, this binding inhibits translocation to the mitochondria or prevents mitochondrial docking.[2]
Cell Research Cell lines Primary cardiac myocytes
Concentrations 1 μmol/L
Incubation Time 2h + 2 to 60 min
Method

Cells culture on chamber slides are treated with P110 and subjected to 2 hour of ischemia followed by 2 to 60 minutes of reoxygenation. At the end of the incubation the cells are washed with cold PBS, fixed in 4% formaldehyde then blocked for 2 hours with 2% normal goat serum in PBS containing 0.1% Triton‐X (blocking buffer). The cells are then incubated overnight at 4°C with antibodies against Tom-20 (1:500). Cells are washed with blocking buffer and incubated for 2 hours with Alexa546-labeled goat antirabbit antibody (1:500). Slides are mounted and imaged by SP5 multiphoton/confocal Laser Scanning Microscope using a ×63 oil immersion objective.
In Vivo
In vivo

P110 treatment inhibits the interaction of fission proteins Fis1/Drp1, decreases mitochondrial fission, and improves bioenergetics in three different rat models of IR, including primary cardiomyocytes, ex vivo heart model, and an in vivo myocardial infarction model.[2]
Animal Research Animal Models Male Wistar rats (270-300 g)
Dosages 0.5 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 2411.78 Formula

C100H179N45O25
CAS No. Unknown SDF --
Smiles CC(C)CC(NC(=O)C(CC(O)=O)NC(=O)CNC(=O)CNC(=O)C(CCCNC(N)=N)NC(=O)C(CCCNC(N)=N)NC(=O)C(CCCNC(N)=N)NC(=O)C(CCC(N)=O)NC(=O)C(CCCNC(N)=N)NC(=O)C(CCCNC(N)=N)NC(=O)C(CCCCN)NC(=O)C(CCCCN)NC(=O)C(CCCNC(N)=N)NC(=O)CNNC(CC1=CC=C(O)C=C1)C=O)C(=O)NC(CC(C)C)C(=O)N2CCCC2C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CO)C(N)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (41.46 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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