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Mdivi-1

Name: Mdivi-1
Brand: Selleck Chemicals
SKU: S7162
Rating: 5 (84 reviews)

Synonyms: Mitochondrial division inhibitor 1

Catalog No.S7162 Purity:99.9%

Mdivi-1 (Mitochondrial division inhibitor 1) is a selective cell-permeable inhibitor of mitochondrial division DRP1 (dynamin-related GTPase) and mitochondrial division Dynamin I (Dnm1) with IC50 of 1-10 μM. Mdivi-1 attenuates mitophagy and enhances apoptosis.

Mdivi-1 Chemical Structure

CAS No. 338967-87-6

Purity & Quality Control

Batch: Purity: 99.9%

99.9

Mdivi-1 Related Products

Related Targets	Dynamin I Dynamin II drp1	Click to Expand
Related Products	Dynasore Dyngo-4a	Click to Expand
Related Compound Libraries	Kinase Inhibitor Library Angiogenesis Related compound Library TGF-beta/Smad compound library Cytoskeletal Signaling Pathway Compound Library Anti-Cardiovascular Disease Compound Library	Click to Expand

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
L1210	Function assay	2.5, 5 and 10 μM	72 h	Mdivi-1 (5 µM) significantly attenuated 0.4 mg/l of cell death in L1210 cells, with the exception of 2.5 and 10 µM of Mdivi-1	28677814
INS-1E	Function assay	50 μM	12 h	treatment with Mdivi-1 significantly inhibited mitochondrial fragmentation and clearly increased the viabilityofpancreatic beta INS-1E cells under hypoxia conditions	29768513
R28	Function assay	5 μM	2 h	mitochondria in R28 cells pretreated with 5 μM Drp1 inhibitor Mdivi-1 retained the average length and appeared as elongated tubular, thread-like networks after irradiation	30538621
HepG2/ADM cells	Cell viability assay	10 μM	1 h	mdivi-1 pretreatment increased B5G1-induced apoptosis and cell death in HepG2/ADM cells	30850585
SH-SY5Y	Function assay	10 μM	30 min	Inhibiting Drp1 protects against CPF-induced intrinsic apoptosis by blocking Bax translocation	26598294
Click to View More Cell Line Experimental Data

Biological Activity

Description

Features

The first selective inhibitor of mitochondrial division dynamins.

Targets

Dnm1 GTPase ^[1]
(Cell-free assay)

1 μM-10 μM

In vitro
In vitro	Mdivi-1 is a cell-permeable quinazolinone compound that inhibits yeast (Dnm1) and mammalian (Drp1) division DRPs (dynamin-related GTPases) and effectively induces mitochondrial fusion into net-like structures in a reversible manner. Cell-free studies indicate that mdivi-1 blocks Dnm1 ATPase activity (IC50<10 μM) and self-assembly by an allosteric modulation-based mechanism. Mdivi-1 is shown to effectively suppress STS- as well as C8-Bid-induced MOMP (Mitochondrial Outer Membrane Permeabilization) in HeLa cultures and in cell-free murine liver mitochondria preparations, respectively, as assessed by cytochrome C release. In cells, mdivi-1 retards apoptosis by inhibiting mitochondrial outer membrane permeabilization. In principle, mivi-1 represents a class of therapeutics for stroke, myocardial infarction, and neurodegenerative diseases. ^[1]
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	p-Chk1 / p-Chk2 / Chk1 / Chk2 / p-ATM / ATM COX-2 / p-Drp1 / Drp1 / Mfn2 / ABCG2 / Oct4		24952704
	Immunofluorescence	β-tubulin		25458053
	Growth inhibition assay	Cell viability Apoptosis		24952704

In Vivo
In vivo	Drp1 and GFAP protein expression is significantly increased in the early neurodegenerative events of ischemic mouse retina. Mdivi-1 treatment blocks apoptotic cell death in ischemic retina, and significantly increases RGC survival at 2 weeks after ischemia. In the normal mouse retina, Drp1 is expressed in the ganglion cell layer (GCL) as well as the inner plexiform layer, the inner nuclear layer (INL), and the outer plexiform layer (OPL). In the GCL, Drp1 immunoreactivity is strong in RGCs. While Drp1 protein expression is increased in the GCL of vehicle-treated ischemic retina at 12 hours. Mdivi-1 treatment does not change this increase of Drp1 protein expression but significantly decreased GFAP protein expression. ^[2]
Animal Research	Animal Models	Male Sprague Dawley rats
	Dosages	3 mg/kg
	Administration	IP injection

References

Chemical Information & Solubility

Molecular Weight	353.22	Formula	C₁₅H₁₀Cl₂N₂O₂S
CAS No.	338967-87-6	SDF	Download Mdivi-1 SDF
Smiles	COC1=C(C=C(C(=C1)N2C(=O)C3=CC=CC=C3NC2=S)Cl)Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 71 mg/mL ( (201.0 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.0mg/ml (14.16mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5% DMSO 1 95% Corn oil	0.8mg/ml (2.26mM)	Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.87mg/ml (5.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 37.4 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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