P110

Catalog No.S9887 Batch:S988701

Technical Data

Formula	C₁₀₀H₁₇₉N₄₅O₂₅
Molecular Weight	2411.78	CAS No.	Unknown
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (41.46 mM)
		Water	100 mg/mL (41.46 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

P110 is a dynamin-related protein 1 (Drp1)-specific inhibitor that binds directly to Drp1.

Targets

Drp1 ^[1]

In vitro

P110 is a Drp1-specific inhibitor that binds directly to Drp1. In some cases, this binding inhibits translocation to the mitochondria or prevents mitochondrial docking.^[2]

In vivo

P110 treatment inhibits the interaction of fission proteins Fis1/Drp1, decreases mitochondrial fission, and improves bioenergetics in three different rat models of IR, including primary cardiomyocytes, ex vivo heart model, and an in vivo myocardial infarction model.^[2]

Protocol (from reference)

Cell Assay:^[2]	Cell lines Primary cardiac myocytes Concentrations 1 μmol/L Incubation Time 2h + 2 to 60 min Method Cells culture on chamber slides are treated with P110 and subjected to 2 hour of ischemia followed by 2 to 60 minutes of reoxygenation. At the end of the incubation the cells are washed with cold PBS, fixed in 4% formaldehyde then blocked for 2 hours with 2% normal goat serum in PBS containing 0.1% Triton‐X (blocking buffer). The cells are then incubated overnight at 4°C with antibodies against Tom-20 (1:500). Cells are washed with blocking buffer and incubated for 2 hours with Alexa546-labeled goat antirabbit antibody (1:500). Slides are mounted and imaged by SP5 multiphoton/confocal Laser Scanning Microscope using a ×63 oil immersion objective.
Animal Study:^{^[2]}	Animal Models Male Wistar rats (270-300 g) Dosages 0.5 mg/kg Administration IP

Cell Assay:^[2]

Cell lines
Primary cardiac myocytes
Concentrations
1 μmol/L
Incubation Time
2h + 2 to 60 min
Method
Cells culture on chamber slides are treated with P110 and subjected to 2 hour of ischemia followed by 2 to 60 minutes of reoxygenation. At the end of the incubation the cells are washed with cold PBS, fixed in 4% formaldehyde then blocked for 2 hours with 2% normal goat serum in PBS containing 0.1% Triton‐X (blocking buffer). The cells are then incubated overnight at 4°C with antibodies against Tom-20 (1:500). Cells are washed with blocking buffer and incubated for 2 hours with Alexa546-labeled goat antirabbit antibody (1:500). Slides are mounted and imaged by SP5 multiphoton/confocal Laser Scanning Microscope using a ×63 oil immersion objective.

Animal Study:^{^[2]}

Animal Models
Male Wistar rats (270-300 g)
Dosages
0.5 mg/kg
Administration
IP

References

Selleck's P110 has been cited by 2 publications

The extracellular matrix integrates mitochondrial homeostasis [ Cell, 2024, S0092-8674(24)00638-X]	PubMed: 38942015
Inhibition of Drp1- Fis1 interaction alleviates aberrant mitochondrial fragmentation and acute kidney injury [ Cell Mol Biol Lett, 2024, 29(1):31]	PubMed: 38439028

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.