Home Apoptosis p53 activator NSC59984 For research use only.

NSC59984

NSC59984 is a p53 pathway activator via induction of mutant p53 protein degradation and p73 activation.

NSC59984 Chemical Structure

NSC59984 Chemical Structure

CAS No. 803647-40-7

Purity & Quality Control

Batch: S810601 DMSO]53 mg/mL]false]Ethanol]53 mg/mL]false]Water]Insoluble]false Purity: 99.77%
99.77

NSC59984 Related Products

Signaling Pathway

p53 Signaling Pathways

p53 Signaling Pathway

Biological Activity

Description NSC59984 is a p53 pathway activator via induction of mutant p53 protein degradation and p73 activation.
Targets
p53 [1]
In vitro
In vitro NSC59984 specifically restores p53 pathway signaling and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. NSC59984 also induces cell death in a panel of cancer cell lines bearing different p53 mutations with little or no genotoxicity in normal cells. [1]
Cell Research Cell lines SW480, DLD-1, HCT116, p53-null HCT116, MRC5, Hop92, RXF393 and Wi38 cells
Concentrations ~100 μM
Incubation Time 72 h
Method Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.
In Vivo
In vivo In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner. [1]

Chemical Information & Solubility

Molecular Weight 265.27 Formula

C12H15N3O4
CAS No. 803647-40-7 SDF Download NSC59984 SDF
Smiles CN1CCN(CC1)C(=O)C=CC2=CC=C(O2)[N+](=O)[O-]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 53 mg/mL ( (199.79 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 53 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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