Home Apoptosis p53 activator NSC 319726 (ZMC1) For research use only.

NSC 319726 (ZMC1)

NSC 319726 (ZMC1) is a p53(R175) mutant reactivator, exhibits growth inhibition in cells expressing mutant p53, with IC50 of 8 nM for p53(R175) mutant, shows no inhibition for p53 wild-type cells.

NSC 319726 (ZMC1) Chemical Structure

NSC 319726 (ZMC1) Chemical Structure

CAS No. 71555-25-4

Purity & Quality Control

Batch: S714901 DMSO]19 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.99%
99.99

NSC 319726 (ZMC1) Related Products

Signaling Pathway

p53 Signaling Pathways

p53 Signaling Pathway

Biological Activity

Description NSC 319726 (ZMC1) is a p53(R175) mutant reactivator, exhibits growth inhibition in cells expressing mutant p53, with IC50 of 8 nM for p53(R175) mutant, shows no inhibition for p53 wild-type cells.
Features An allele-specific p53 mutant reactivator.
Targets
p53 (R175) [1]
8 nM
In vitro
In vitro NSC319726 is a p53(R175) mutant reactivator, exhibits growth inhibition in cells expressing mutant p53, with IC50 of 8 nM for p53(R175) mutant, shows no inhibition for p53 wild-type cells, shows 10- to 100-fold selectivity over the other hotspot p53 mutants. NSC319276 induces p53(R175)-mutant-dependent apoptosis. NSC319726 treatment induces a WT-like conformational change in the p53(R175) mutant protein that restores sequence-specific p53 transcription. The activity of NSC319726 depends on the zinc ion chelating properties of the compound as well as redox changes. [1]
Kinase Assay MTS
5,000 cells of TOV112D cells (5,000 cells/well, in 100 ml culture) are cultured in 96-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compounds. The growth is measured by MTS reagent and Victor Plate reader instrument after incubation for 3 days.
Cell Research Cell lines MEF with p53 mutations
Concentrations ~10 μM
Incubation Time 3 days
Method Viability assays are done. The cells (5×104 cells/well, in 1 ml culture) are cultured in a 12-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compound. The growth is measured by Guava ViaCount reagent and Guava PCA instrument after incubation for 3 days.
In Vivo
In vivo NSC319726 (5mg/kg, 7days) exhibits greater toxicity for p53(R172H/R172H) mice, with only 30% survival, as compared to 100% survival for p53+/+ and p53-/- mice. NSC319726 inhibits the growth of TOV112D-p53(R175H) xenografts, but not H460 (p53+/+) and MDAMB468-p53(R273W) xenografts. [1]
Animal Research Animal Models p53+/+, p53-/- and p53R172H knockin mice
Dosages 5-10 mg/kg/day, up to 7 days
Administration i.p.

Chemical Information & Solubility

Molecular Weight 234.32 Formula

C11H14N4S
CAS No. 71555-25-4 SDF Download NSC 319726 (ZMC1) SDF
Smiles CC(=NNC(=S)N1CCC1)C2=CC=CC=N2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 19 mg/mL ( (81.08 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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