Home Metabolism phosphatase inhibitor NSC45586 For research use only.

NSC45586

NSC45586 is an inhibitor of PHLPP (Pleckstrin homology domain and leucine-rich repeat protein phosphatase), which is selective for PHLPP1 and PHLPP2.

NSC45586 Chemical Structure

NSC45586 Chemical Structure

CAS No. 6300-44-3

Purity & Quality Control

Batch: S445101 DMSO]82 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 98.18%
98.18

NSC45586 Related Products

Biological Activity

Description NSC45586 is an inhibitor of PHLPP (Pleckstrin homology domain and leucine-rich repeat protein phosphatase), which is selective for PHLPP1 and PHLPP2.
Targets
PHLPP [1]
In vitro
In vitro

PH domain and leucine rich repeat phosphatase (Phlpp) inhibitor NSC45586 increases Pth1r mRNA and protein expression in ATDC5 cells and IMCs within 24 hours. [2]
Cell Research Cell lines ATDC5 cells, Primary immature chondrocytes (IMCs)
Concentrations 25 μM
Incubation Time 24 h
Method

ATDC5 cells were seeded at a density of 5 x 105 cells/well in a six-well plate and incubated overnight to allow for cell adhesion in DMEM supplemented with 10% FBS and 100 units/mL penicillin, 100 μg/mL streptomycin, and 0.25 μg/mL Amphotericin B (1% antibiotic/antimycotic). Phlppi including NSC45586 (25 μM) treatments were added. Primary chondrocytes were plated and allowed to adhere overnight as described above. Actinomycin D (5μM) was added for six hours and then replaced with medium containing Phlppi including NSC45586 or vehicle for 24 hours. Cells were collected for RNA or protein extraction.

Chemical Information & Solubility

Molecular Weight 412.38 Formula

C20H17N6NaO3
CAS No. 6300-44-3 SDF --
Smiles [Na+].CC1=CC(=C(N)C=C1N)N=NC2=CC=C(C=C2)N=NC3=CC(=C(O)C=C3)C([O-])=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 82 mg/mL ( (198.84 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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