Home Metabolism phosphatase inhibitor β-Glycerophosphate sodium salt hydrate For research use only.

β-Glycerophosphate sodium salt hydrate

β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.

β-Glycerophosphate sodium salt hydrate Chemical Structure

β-Glycerophosphate sodium salt hydrate Chemical Structure

CAS No. 13408-09-8

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β-Glycerophosphate sodium salt hydrate Related Products

Biological Activity

Description β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.
Targets
phosphatase [1]
In vitro
In vitro β-glycerophosphate accelerates in vitro calcification by BVSMC and induces extensive calcium deposition in a manner analogous to in vitro mineralization by osteoblasts. β-glycerophosphate can increase ALP activity in osteoblasts[2].
Cell Research Cell lines BVSMC (bovine vascular smooth muscle cells)
Concentrations 10 mmol/L
Incubation Time 14 d
Method BVSMC are cultured in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS (growing medium). After confluence, the cells are inoculated in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS in the presence of 10 mmol/L β-glycerophosphate, 10-7 mol/L insulin, and 50 μg/mL of ascorbic acid for 14 days (calcification medium). The medium is replaced with fresh medium every 3 days. In certain experiments, only β-glycerophosphate is used to induce BVSMC calcification. Moreover, in the time-course experiments, before being cultured in calcification medium (defined as day 0), cells are selected to be used as controls.

Chemical Information & Solubility

Molecular Weight 306.11 Formula

C3H9O6P.5H2O.2Na
CAS No. 13408-09-8 SDF Download β-Glycerophosphate sodium salt hydrate SDF
Smiles C(C(CO)OP(=O)([O-])[O-])O.O.O.O.O.O.[Na+].[Na+]
Storage (From the date of receipt)

In vitro
Batch:

Water : 61 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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