β-Glycerophosphate sodium salt hydrate

Catalog No.S3620 Batch:S362003

Technical Data

Formula	C₃H₉O₆P.5H₂O.2Na
Molecular Weight	306.11	CAS No.	13408-09-8
Solubility (25°C)*	In vitro	Water	61 mg/mL (199.27 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.

Targets

phosphatase ^[1]

In vitro

β-glycerophosphate accelerates in vitro calcification by BVSMC and induces extensive calcium deposition in a manner analogous to in vitro mineralization by osteoblasts. β-glycerophosphate can increase ALP activity in osteoblasts^[2].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines BVSMC (bovine vascular smooth muscle cells) Concentrations 10 mmol/L Incubation Time 14 d Method BVSMC are cultured in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS (growing medium). After confluence, the cells are inoculated in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS in the presence of 10 mmol/L β-glycerophosphate, 10^-7 mol/L insulin, and 50 μg/mL of ascorbic acid for 14 days (calcification medium). The medium is replaced with fresh medium every 3 days. In certain experiments, only β-glycerophosphate is used to induce BVSMC calcification. Moreover, in the time-course experiments, before being cultured in calcification medium (defined as day 0), cells are selected to be used as controls.

Cell Assay:^{^[2]}

Cell lines
BVSMC (bovine vascular smooth muscle cells)
Concentrations
10 mmol/L
Incubation Time
14 d
Method
BVSMC are cultured in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS (growing medium). After confluence, the cells are inoculated in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS in the presence of 10 mmol/L β-glycerophosphate, 10^-7 mol/L insulin, and 50 μg/mL of ascorbic acid for 14 days (calcification medium). The medium is replaced with fresh medium every 3 days. In certain experiments, only β-glycerophosphate is used to induce BVSMC calcification. Moreover, in the time-course experiments, before being cultured in calcification medium (defined as day 0), cells are selected to be used as controls.

References

Selleck's β-Glycerophosphate sodium salt hydrate has been cited by 12 publications

Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis [ Nat Commun, 2024, 15(1):2384]	PubMed: 38493144
A metabolic reprogramming-related gene signature correlates with prognosis and proliferation of BLCA [ Discov Oncol, 2024, 15(1):338]	PubMed: 39115575
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644]	PubMed: 36993303
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644]	PubMed: 36993303
Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis [ Cell Mol Immunol, 2022, 19(10):1153-1167]	PubMed: 36050478
Sphingosine kinase 1 promotes tumor immune evasion by regulating the MTA3-PD-L1 axis [ Cell Mol Immunol, 2022, 19(10):1153-1167]	PubMed: 36050478
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]	PubMed: 34997030
A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma [ Mol Med Rep, 2022, 26(2)255]	PubMed: 35703357
MiR-140-5p upregulation suppressed β-glycerophosphate-induced vascular smooth muscle cell calcification via targeting TLR4 [ Immunopharmacol Immunotoxicol, 2022, 1-11]	PubMed: 35272550
Identification of a Three-Glycolysis-Related lncRNA Signature Correlated With Prognosis and Metastasis in Clear Cell Renal Cell Carcinoma [ Front Med (Lausanne), 2021, 8:777507]	PubMed: 35083240

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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