Home Immunology & Inflammation NLRP3 inhibitor MCC950 Sodium For research use only.

MCC950 Sodium

Synonyms: CP-456773 Sodium, CRID3 Sodium

MCC950 Sodium is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.

MCC950 Sodium Chemical Structure

MCC950 Sodium Chemical Structure

CAS No. 256373-96-3

Purity & Quality Control

MCC950 Sodium Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse BMDM cells Function assay 30 mins Inhibition of NLRP3 in mouse BMDM cells preincubated for 30 mins followed by ATP addition by ELISA analysis. IC50=0.0075 μM 26422006
mouse BMDM cells Function assay 30 mins Inhibition of NLRP3 inflammasome activation in LPS-stimulated mouse BMDM assessed as reduction in NLRP3 inflammasome-induced IL-1beta production preincubated for 30 mins followed by NLRP3 stimulation with ATP by ELISA. IC50=0.0075 μM 27994733
INS-1 cells Function assay 0.01 μM 1 h MCC950 inhibited not only inflammasome activation but also Ang II-induced IL-1β elevation and cell apoptosis in INS-1 cells. 30939192
16HBE cells Cell viability assay 0.01, 0.1, 1, 10, 100 μM 24, 48, 72h 100μM MCC950 functionally alleviated silica-induced activation of NLRP3 inflammasome in 16 HBE cells. 29258746
THP-1 monocytes Function assay 1 μM 30 mins Pretreatment of THP‐1 monocytes for 30 min with this compound resulted in a significant reduction in IL‐1β release, but not pyroptosis upon transfection with LPS. 26173988
macrophages Function assay 30 mins Inhibition of NLRP3 in human monocyte derived macrophages assessed as reduction in LPS-induced IL-1beta production incubated for 30 mins followed by nigericin sodium salt stimulation for 2 hrs post LPS challenge for 3 hrs by ELISA, IC50 = 0.005 μM. 29338910
macrophages Function assay Inhibition of NLRP3 in human monocyte derived macrophages assessed as reduction in LPS-induced IL-1beta production by ELISA, IC50 = 0.008 μM. 29338910
Click to View More Cell Line Experimental Data

Biological Activity

Description MCC950 Sodium is a potent, selective inhibitor of NLRP3 with IC50 of 7.5 nM in BMDMs; but not the AIM2, NLRC4 or NLRP1 inflammasomes.
Targets
NLRP3 [1]
(in BMDM (bone marrow derived macrophages))		 NLRP3 [1]
(in HMDM)
7.5nM 8.1 nM
In vitro
In vitro

MCC950 inhibits canonical and noncanonical NLRP3 activation in BMDM and HMDM cells at IC50 of 7.5 and 8.1nM. MCC950 specifically inhibits activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.[1]
Cell Research Cell lines H9c2 cardiomyocyte cells
Concentrations 7 nM
Incubation Time 24 h
Method

Cardiomyocytes were treated with NLRP3 inhibitor MCC950 for 24 h.
Experimental Result Images Methods Biomarkers Images PMID
Western blot NLRP3 / Casp-1 / BMI1 / ALDH1 / CD44 28865486
Growth inhibition assay Cell viability 27670879
Immunofluorescence ALDH1 / BIM1 / CD44 28865486
ELISA IL-1β 28726778
In Vivo
In vivo

MCC950 reduces interleukin-1β (IL-1β) production and attenuates the severity of experimental autoimmune encephalomyelitis (EAE). Furthermore, MCC950 treatment rescues neonatal lethality in a mouse model of CAPS and is active in ex vivo samples from individuals with Muckle-Wells syndrome. [1]
Animal Research Animal Models C57BL/6 mice
Dosages 50 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 426.46 Formula

C20H23N2O5S.Na
CAS No. 256373-96-3 SDF Download MCC950 Sodium SDF
Smiles CC(C)(C1=COC(=C1)S(=O)(=O)[N-]C(=O)NC2=C3CCCC3=CC4=C2CCC4)O.[Na+]
Storage (From the date of receipt)

In vitro
Batch:

Water : 43 mg/mL

Ethanol : 43 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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