Home Immunology & Inflammation NLRP3 inhibitor INF39 For research use only.

INF39

INF39 is a nontoxic, irreversible NLRP3 inhibitor that counteracts NLRP3 activation through direct irreversible interaction with NLRP3 and partial inhibition of LPS-driven pro-inflammatory gene expression.

INF39 Chemical Structure

INF39 Chemical Structure

CAS No. 866028-26-4

Purity & Quality Control

INF39 Related Products

Biological Activity

Description INF39 is a nontoxic, irreversible NLRP3 inhibitor that counteracts NLRP3 activation through direct irreversible interaction with NLRP3 and partial inhibition of LPS-driven pro-inflammatory gene expression.
Targets
NLRP3 [1]
In vitro
In vitro INF39 inhibits NLRP3 ATPase in a concentration-dependent manner and prevents pyroptosis of THP-1 cells. It is able to efficiently inhibit NLRP3-dependent IL-1β release and reduce caspase-1 activation and pyroptosis (measured as extracellular LDH) in macrophages (BMDMs)[1].
Cell Research Cell lines THP-1 cells
Concentrations 0.1−100 μM
Incubation Time 72 h
Method

Human THP-1 cells were exposed to increasing concentrations of the synthesized compounds (0.1−100 μM, 72 h), and then cell viability was evaluated by the MTT assay
In Vivo
In vivo Oral Administration of INF39 reduces systemic and colonic inflammation in rats treated with 2,4-Dinitrobenzenesulfonic acid. Rats treated with INF39 display a significant reduction of macroscopic damage score. Oral administration of INF39 reduces colonic myeloperoxidase, IL-1β, and TNF Levels in DNBS-treated rats. INF39 can exert beneficial effects on colitis, by reducing MPO, IL-1β, and TNF pro inflammatory cytokine levels in colonic tissues from inflamed rats, thus suggesting that the blockade of NLPR3 activation could represent a suitable pharmacological target for the management of intestinal inflammation. INF39, following oral administration, is stable and is absorbed into the intestinal epithelium, where it can act locally and generate the nontoxic metabolites[1].
Animal Research Animal Models Male Sprague−Dawley rats
Dosages 12.5, 25.0, 50.0 mg/kg/day
Administration oral administration

Chemical Information & Solubility

Molecular Weight 224.68 Formula

C12H13ClO2
CAS No. 866028-26-4 SDF Download INF39 SDF
Density 1.1 g/mL
Smiles CCOC(=O)C(=C)CC1=CC=CC=C1Cl
Storage (From the date of receipt) 2 years -80°C liquid

In vitro
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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