Home Immunology & Inflammation Interleukins inhibitor EC330 For research use only.

EC330

EC330 is a steroidal inhibitor of leukemia inhibitory factor (LIF, an interleukin 6 class cytokine). EC330 inhibits the LIF/LIF-R signaling and blocks the promoting effects of LIF on growth and migration of cancer cells.

EC330 Chemical Structure

EC330 Chemical Structure

CAS No. 2016795-77-8

Purity & Quality Control

EC330 Related Products

Biological Activity

Description EC330 is a steroidal inhibitor of leukemia inhibitory factor (LIF, an interleukin 6 class cytokine). EC330 inhibits the LIF/LIF-R signaling and blocks the promoting effects of LIF on growth and migration of cancer cells.
Targets
LIF [1]
In vitro
In vitro

EC330, a potential LIF inhibitor, inhibits the activation of STAT3, PI3K/AKT, and mTOR signaling pathways by LIF, preferentially inhibits the proliferation of cancer cells with LIF overexpression, also inhibits LIF function in promoting migration of cancer cells.[1]
Cell Research Cell lines MCF7, MDA-MB231 cell lines
Concentrations --
Incubation Time 24 h
Method

The effect of EC330 on viability of cells with or without ectopic LIF expression was assessed in cells treated with different concentrations of EC330 for 24 h. Cell viability was determined by   the Vi-CELL counter that performs a trypan blue exclusion method. Cell proliferation was   determined by counting cell numbers daily for four days.
In Vivo
In vivo

EC330 inhibits multi-functional cytokine LIF function in promoting tumor growth in vivo.[1]
Animal Research Animal Models BALB/c nude mice model of subcutaneous xenograft tumors formed by MDA-MB231 cells with ectopic LIF expression
Dosages 1 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 462.57 Formula

C30H32F2O2
CAS No. 2016795-77-8 SDF --
Smiles CC12CC(C3=C4CCC(=O)C=C4CCC3C1CCC2(C(C#C)(F)F)O)C5=CC=C(C=C5)C6CC6
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Ethanol : 93 mg/mL

DMSO : 12 mg/mL ( (25.94 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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