EC330

Catalog No.S0472 Batch:S047202

Technical Data

Formula

C₃₀H₃₂F₂O₂

Molecular Weight

462.57

CAS No.

2016795-77-8

Solubility (25°C)*

In vitro

Ethanol

93 mg/mL (201.05 mM)

DMSO

23 mg/mL (49.72 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	1.15mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 23 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil	1.15mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 23 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

EC330 is a steroidal inhibitor of leukemia inhibitory factor (LIF, an interleukin 6 class cytokine). EC330 inhibits the LIF/LIF-R signaling and blocks the promoting effects of LIF on growth and migration of cancer cells.

Targets

LIF ^[1]

In vitro

EC330, a potential LIF inhibitor, inhibits the activation of STAT3, PI3K/AKT, and mTOR signaling pathways by LIF, preferentially inhibits the proliferation of cancer cells with LIF overexpression, also inhibits LIF function in promoting migration of cancer cells.^[1]

In vivo

EC330 inhibits multi-functional cytokine LIF function in promoting tumor growth in vivo.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MCF7, MDA-MB231 cell lines Concentrations -- Incubation Time 24 h Method The effect of EC330 on viability of cells with or without ectopic LIF expression was assessed in cells treated with different concentrations of EC330 for 24 h. Cell viability was determined by the Vi-CELL counter that performs a trypan blue exclusion method. Cell proliferation was determined by counting cell numbers daily for four days.
Animal Study:^{^[1]}	Animal Models BALB/c nude mice model of subcutaneous xenograft tumors formed by MDA-MB231 cells with ectopic LIF expression Dosages 1 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MCF7, MDA-MB231 cell lines
Concentrations
--
Incubation Time
24 h
Method
The effect of EC330 on viability of cells with or without ectopic LIF expression was assessed in cells treated with different concentrations of EC330 for 24 h. Cell viability was determined by the Vi-CELL counter that performs a trypan blue exclusion method. Cell proliferation was determined by counting cell numbers daily for four days.

Animal Study:^{^[1]}

Animal Models
BALB/c nude mice model of subcutaneous xenograft tumors formed by MDA-MB231 cells with ectopic LIF expression
Dosages
1 mg/kg
Administration
i.p.

References

[1] Xuetian Yue, et al. J Mol Cell Biol. 2020 Jul 3;12(6):477-480.

Selleck's EC330 has been cited by 2 publications

CCL3 predicts exceptional response to TGFβ inhibition in basal-like pancreatic cancer enriched in LIF-producing macrophages [ NPJ Precis Oncol, 2024, 8(1):246]	PubMed: 39478186
PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer [ Br J Cancer, 2021, 10.1038/s41416-021-01631-3]	PubMed: 34815524

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.