Home Immunology & Inflammation Interleukins inhibitor - Immunology & Inflammation related modulator Ossirene (AS101) For research use only.

Ossirene (AS101)

Ossirene (AS101), a potent in vitro and in vivo immunomodulator, is a novel inhibitor of IL-1beta converting enzyme.

Ossirene (AS101) Chemical Structure

Ossirene (AS101) Chemical Structure

CAS No. 106566-58-9

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Ossirene (AS101) Related Products

Biological Activity

Description Ossirene (AS101), a potent in vitro and in vivo immunomodulator, is a novel inhibitor of IL-1beta converting enzyme.
Targets
IL-1β converting enzyme [1]
In vitro
In vitro Treatment of caspase-1 (interleukin-1 [IL-1] converting enzyme [ICE]) with AS101 inhibits its enzymatic activity in a dose-dependent manner. Moreover, AS101 treatment causes a significant reduction in the active form of IL-18 and IL-1 in peripheral blood mononuclear cells (PBMC) and in human HaCat keratinocytes. The inhibitory effect of AS101 does not involve nitric oxide (NO) or interferon- (IFN- ), two possible regulators of IL-18 production, and does not occur at the mRNA level. It may exert effects through a posttranscriptional mechanism[1]. AS101 induced SIRT1 expression in a dose dependent manner in three different cell lines, HEK293, HL-60 and Rin-5f. Incubation of HEK293 and HL-60 cell lines with AS101 (0.1-2.5μg/ml) resulted in a dose dependent reduction in PPARγ protein expression relative to the control cells, and the reduction of PPARγ expression is in parallel to increased SIRT1 expression[2].
Cell Research Cell lines Human peripheral blood mononuclear cells (PBMC)
Concentrations 0.01, 0.05, 0.5, 1, 1.5, 2 μg/mL
Incubation Time 24 h
Method

In vitro, PBMCs were first treated with various concentrations of AS101, and after 1 h SAC (10-3 v/v) was added. After 24 h, supernatants were collected and evaluated for cytokine content.
In Vivo
In vivo AS101 downregulates IL-18 and IL-1 serum levels in a mouse model of lipopolysaccharide (LPS)-induced sepsis, resulting in increased survival. It has also been shown to be protective against lethal and sublethal effects of irradiation and chemotherapy. In a model of cecal ligation and puncture (CLP)-induced sepsis in mice, AS101 also exerted beneficial effects[1]. AS101 treated rats showed a large increase in SIRT1 protein levels in the liver and kidney extracts of rats treated with AS101. AS101 prevents development of insulin resistance in vivo. AS101 affects SIRT1 related metabolic pathways by changing the insulin levels. AS101 treatment prevents hyperglycemia associated with T2D(type 2 diabetes) and some of the symptoms of the disease in the HFD(high fat diet)+STZ(Streptozotocin) rat model[2].
Animal Research Animal Models c57BL/6J and BALB/c mice
Dosages 10 μg/mouse
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01943630 Unknown status
External Genital Warts
BioMAS Ltd
 January 2015 Phase 2
NCT01010373 Suspended
Acute Myeloid Leukemia|Myelodysplastic Syndrome
BioMAS Ltd
 January 2015 Phase 2

Chemical Information & Solubility

Molecular Weight 312.05 Formula

C2H4Cl3O2Te.H4N
CAS No. 106566-58-9 SDF Download Ossirene (AS101) SDF
Smiles C1CO[Te](O1)(Cl)(Cl)Cl.[NH4+]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 62 mg/mL ( (198.68 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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