Home Proteases Antineoplastic and Immunosuppressive Antibiotics inhibitor - MMP inhibitor Doxycycline Hyclate For research use only.

Doxycycline Hyclate

Synonyms: WC2031

Doxycycline Hyclate is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).

Doxycycline Hyclate Chemical Structure

Doxycycline Hyclate Chemical Structure

CAS No. 24390-14-5

Purity & Quality Control

Doxycycline Hyclate Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SW1353 Function assay 50 uM Inhibition of IL-1-beta-induced MMP13 production in human SW1353 cells at 50 uM 17267227
K-12 BW25113 Bactericidal assay 24 hrs Bactericidal activity against yafQ gene-deficient Escherichia coli K-12 BW25113 biofilm assessed as log reduction of viable cells after 24 hrs 19307375
K-12 BW25113 Bactericidal assay 24 hrs Bactericidal activity against Escherichia coli K-12 BW25113 biofilm harboring pCA24N ptac::yafQ plasmid assessed as log reduction of viable cells after 24 hrs pretreated with 5 uM of IPTG for 4 hrs 19307375
K562 Cytotoxicity assay 72 hrs Cytotoxicity against human K562 cells after 72 hrs by flow cytometry, IC50 = 15 μM. 19482476
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50 = 20 μM. 19482476
THP1 Function assay Selectivity index, ratio of IC50 for human THP1 cells to IC50 for Plasmodium falciparum, IC50 = 3.1 μM. 19748781
THP1 Cytotoxicity assay 72 hrs Cytotoxicity against human THP1 cells after 72 hrs by propidium iodide staining-based flow cytometry, IC50 = 20 μM. 19748781
K562 Cytotoxicity assay 72 hrs Cytotoxicity against human K562 cells after 72 hrs by flow cytometry, IC50 = 15 μM. 19926173
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, IC50 = 20 μM. 19926173
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay, CC50 = 20 μM. 21741131
Neuro2a Function assay 1 uM Decrease in 7-DHC levels in Dhcr7-deficient mouse Neuro2a cells at 1 uM by LC-MS/GC-MS analysis 26789657
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells measured after 72 hrs by MTT assay, CC50 = 20 μM. 27155463
HepG2 Cytotoxicity assay 72 hrs Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay, CC50 = 20 μM. 27654395
CD Antibacterial assay 25 ug/ml 72 hrs Antibacterial activity against Rickettsia prowazekii str. Breinl infected in CD rat primary pulmonary vascular endothelial cells assessed as bacterial shape change at 25 ug/ml measured 72 hrs post infection by Hoechst 33258/phalloidin staining based fluor 28089350
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
skeletal myoblast cells Cytotoxicity assay DNDI: Cytotoxicity in Vitro, 72 hour, in rat skeletal myoblast cells, IC50 = 14.49 μM. ChEMBL
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Biological Activity

Description Doxycycline Hyclate is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).
Targets
MMP [6]
In vitro
In vitro

Doxycycline hyclate 10% (DOXY) has significant toxicity effects on osteoblast cells compared to the control group with a mean cell viability of 84%. Doxycycline hyclate 10% (DOXY) results in a significantly higher alkaline phosphatase activity (approximately 56%) than the control in osteoblasts. Doxycycline hyclate 10% (DOXY) seems to slightly altered the morphology of the osteoblasts. [1]
Cell Research Cell lines MEFs
Concentrations 100 ng/ml
Incubation Time 12 h
Method

Cells were treated with various concentrations of drug.
Experimental Result Images Methods Biomarkers Images PMID
Western blot AKT / BCL6 / Cyclin E / HDAC2 / HDAC3 / NEMO / TYK2 / RIPK1 HSP70 / HSP90 pATM / ATM 26142707
Immunofluorescence E-cadherin / Vimentin 29285218
In Vivo
In vivo

Doxycycline hyclate is an effective treatment for acute E. canis infection in dogs. [2]

Doxycycline hyclate (5 mg/kg, orally) decreases the collagenase activity in corneas obtained from rabbits. Doxycycline hyclate (5 mg/kg, orally) promotes corneal reepithelialization in the rabbit alkali-burn model, a result, perhaps, of the drug's ability to inhibit excessive collagenase activity. [3]

Doxycycline hyclate results in 43% of prophylaxis in orally treated mice, in contrast, the sustained-release doxycycline hyclate completely protects mice from infection and resultant pathology. [4]

Doxycycline hyclate results in bioavailability of 70%, Cmin of 1 mg/L, Cmax of 2.3 mg/L and elimination half-life of 7 hours in calves with immature rumen function. [5]

Chemical Information & Solubility

Molecular Weight 512.94 Formula

C22H24N2O8.1/2C2H6O.HCl.1/2H2O
CAS No. 24390-14-5 SDF Download Doxycycline Hyclate SDF
Smiles CCO.CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.O.Cl.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (194.95 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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