Home Metabolism Fatty Acid Synthase inhibitor - Other activator - CPT agonist C75 For research use only.

C75

C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) , a more stable synthetic mimic of cerulenin, inhibits fatty-acid synthase (FAS/FASN) with an IC50 of 15.53 μM and has potential therapeutic effects in several cancer models. C75 is also a potent CPT1A activator.

C75 Chemical Structure

C75 Chemical Structure

CAS No. 218137-86-1

Purity & Quality Control

Batch: S981901 DMSO]51 mg/mL]false]Ethanol]51 mg/mL]false]Water]Insoluble]false Purity: 99.93%
99.93

C75 Related Products

Biological Activity

Description C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) , a more stable synthetic mimic of cerulenin, inhibits fatty-acid synthase (FAS/FASN) with an IC50 of 15.53 μM and has potential therapeutic effects in several cancer models. C75 is also a potent CPT1A activator.
Targets
FAS/FASN [1]
15.53 μM
In vitro
In vitro

C75, the competitive irreversible fatty acid synthase (FASN) inhibitor, possesses radiosensitizing activity in prostate cancer cell lines that is affected by the availability of fatty acids, also increases expression of fatty acid transporter proteins FATP1 and CD36.[3]
Cell Research Cell lines PC3, UVW and LNCaP cell lines, SK-N-BE(2c) and MCF7 cells
Concentrations 35 µM
Incubation Time 24 h
Method

Cells are seeded in 25 cm2 flasks at 105 cells/flask. When cultures are in exponential growth phase, medium is removed and replaced with fresh medium (including 1% or 10% serum) containing C75 (35 mM) and simultaneous administration of x-rays (2 Gy). Cells are then incubated for 24 h at 37℃ in 5% CO2. After treatment, cells are counted and seeded for clonogenic survival assay.  
In Vivo
In vivo

C75, an alpha-methylene-gamma-butyrolactone, reduces adipose tissue and fatty liver in treated DIO mice, despite high levels of malonyl-CoA.[4]
Animal Research Animal Models Diet-induced obese mice model
Dosages 20, 15, 10, 15, 15 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 254.32 Formula

C14H22O4
CAS No. 218137-86-1 SDF --
Smiles CCCCCCCCC1OC(=O)C(=C)C1C(O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 51 mg/mL ( (200.53 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 51 mg/mL

Water : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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