Home Metabolism Fatty Acid Synthase inhibitor C75 trans For research use only.

C75 trans

Synonyms: trans-C75, (±)-C75

C75 trans (trans-C75, (±)-C75) is a novel, potent synthetic inhibitor of fatty acid synthase (FAS/FASN) with IC50 of 35 μM and 50 μM in clonogenic assays and spheroid growth assay, respectively. C75 is used as a tool for studying fatty acid synthesis in metabolic disorders and cancer.

C75 trans Chemical Structure

C75 trans Chemical Structure

CAS No. 191282-48-1

Purity & Quality Control

C75 trans Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MA104 Antiviral assay 24 hrs Antiviral activity against Rotavirus SA11 in MA104 cells assessed as inhibition of viral replication after 24 hrs by immunofluorescent assay and Western blotting analysis, ED50 = 21.2 μM. 22365411
MA104 Cytotoxicity assay 24 hrs Cytotoxicity against monkey MA104 cells after 24 hrs, TD50 = 28.5 μM. 22365411
A375 Function assay Inhibition of FASN in human A375 cells, IC50 = 32.43 μM. 21726077
KB Cytotoxicity assay Cytotoxicity in human KB cells, IC50 = 36.5 μM. 21504156
Click to View More Cell Line Experimental Data

Biological Activity

Description C75 trans (trans-C75, (±)-C75) is a novel, potent synthetic inhibitor of fatty acid synthase (FAS/FASN) with IC50 of 35 μM and 50 μM in clonogenic assays and spheroid growth assay, respectively. C75 is used as a tool for studying fatty acid synthesis in metabolic disorders and cancer.
Targets
FASN [1]
(clonogenic assay)		 FASN [1]
(spheroid growth assay)
35 μM 50 μM
In vitro
In vitro

C75 is toxic to prostate cancer cells. C75 reduces cell migration, while enhances radiation-induced clonogenic kill and enhances the spheroid growth delay induced by radiation exposure. Apoptosis induction by radiation exposure is enhanced by C75 treatment.[1] C75 inhibits fatty acid synthesis and growth of established cancer cell lines from neu-N transgenic mice. C75 reduces the proliferation and expression of FAS, neu, and related genes in mammary epithelium.[2]
Cell Research Cell lines PC3 cells, LNCaP cells
Concentrations 5 μM, 25 μM, 35 μM, 50 μM
Incubation Time 3 h, 6 h, 24 h
Method

PC3 cells are seeded in 25 cm2 flasks at 105 cells/flask. When cultures are in exponential growth phase, media is removed and replaced with fresh drug-containing media. Cells are then incubated for 24 h at 37℃ in 5% CO2. To determine optimal sequencing of therapeutic agents, three different combination treatment schedules are assessed: 1. radiation and C75 administered simultaneously; 2. radiation administered 24 h before C75; and 3. radiation administered 24 h after C75. After treatment, cells are seeded for clonogenic survival assay.
In Vivo
In vivo

C75 inhibits fatty acid synthesis and in vivo mammary cancer development. C75 treatment prevents mammary cancer development and retards mammary development in neu-N transgenic mice.[2]
Animal Research Animal Models neu-N transgenic mice, FVB/N mice
Dosages 30mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 254.32 Formula

C14H22O4
CAS No. 191282-48-1 SDF --
Smiles CCCCCCCCC1C(C(=C)C(=O)O1)C(=O)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 51 mg/mL ( (200.53 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 26 mg/mL

Water : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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