C75

Catalog No.S9819 Batch:S981901

Technical Data

Formula

C₁₄H₂₂O₄

Molecular Weight

254.32

CAS No.

218137-86-1

Solubility (25°C)*

In vitro

DMSO

51 mg/mL (200.53 mM)

Ethanol

51 mg/mL (200.53 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 Corn oil	3.0mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) , a more stable synthetic mimic of cerulenin, inhibits fatty-acid synthase (FAS/FASN) with an IC50 of 15.53 μM and has potential therapeutic effects in several cancer models. C75 is also a potent CPT1A activator.

Targets

FAS/FASN ^[1]

15.53 μM

In vitro

C75, the competitive irreversible fatty acid synthase (FASN) inhibitor, possesses radiosensitizing activity in prostate cancer cell lines that is affected by the availability of fatty acids, also increases expression of fatty acid transporter proteins FATP1 and CD36.^[3]

In vivo

C75, an alpha-methylene-gamma-butyrolactone, reduces adipose tissue and fatty liver in treated DIO mice, despite high levels of malonyl-CoA.^[4]

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines PC3, UVW and LNCaP cell lines, SK-N-BE(2c) and MCF7 cells Concentrations 35 µM Incubation Time 24 h Method Cells are seeded in 25 cm₂ flasks at 10₅ cells/flask. When cultures are in exponential growth phase, medium is removed and replaced with fresh medium (including 1% or 10% serum) containing C75 (35 mM) and simultaneous administration of x-rays (2 Gy). Cells are then incubated for 24 h at 37℃ in 5% CO2. After treatment, cells are counted and seeded for clonogenic survival assay.
Animal Study:^{^[4]}	Animal Models Diet-induced obese mice model Dosages 20, 15, 10, 15, 15 mg/kg Administration i.p.

Cell Assay:^{^[3]}

Cell lines
PC3, UVW and LNCaP cell lines, SK-N-BE(2c) and MCF7 cells
Concentrations
35 µM
Incubation Time
24 h
Method
Cells are seeded in 25 cm₂ flasks at 10₅ cells/flask. When cultures are in exponential growth phase, medium is removed and replaced with fresh medium (including 1% or 10% serum) containing C75 (35 mM) and simultaneous administration of x-rays (2 Gy). Cells are then incubated for 24 h at 37℃ in 5% CO2. After treatment, cells are counted and seeded for clonogenic survival assay.

Animal Study:^{^[4]}

Animal Models
Diet-induced obese mice model
Dosages
20, 15, 10, 15, 15 mg/kg
Administration
i.p.

References

[4] Thupari JN, et al. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9498-502.

+ Expand

Selleck's C75 has been cited by 4 publications

Modulation of host lipid metabolism by virus infection leads to exoskeleton damage in shrimp [ PLoS Pathog, 2024, 20(5):e1012228]	PubMed: 38739679
Restoration of CPT1A-mediated fatty acid oxidation in mesothelial cells protects against peritoneal fibrosis [ Theranostics, 2023, 13(13):4482-4496]	PubMed: 37649600
Restoration of CPT1A-mediated fatty acid oxidation in mesothelial cells protects against peritoneal fibrosis [ Theranostics, 2023, 10.7150/thno.84921]	PubMed: 37649600
Enterovirus A71 utilizes host cell lipid β-oxidation to promote its replication [ Front Microbiol, 2022, 13:961942]	PubMed: 36246276

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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