Home Epigenetics Epigenetic Reader Domain inhibitor Bromosporine For research use only.

Bromosporine

Bromosporine is a broad spectrum inhibitor for bromodomains with IC50 of 0.41 μM, 0.29 μM, 0.122 μM and 0.017 μM for BRD2, BRD4, BRD9 and CECR2, respectively.

Bromosporine Chemical Structure

Bromosporine Chemical Structure

CAS No. 1619994-69-2

Purity & Quality Control

Bromosporine Related Products

Signaling Pathway

Epigenetic Reader Domain Signaling Pathways

Epigenetic Reader Domain Signaling Pathway

Biological Activity

Description Bromosporine is a broad spectrum inhibitor for bromodomains with IC50 of 0.41 μM, 0.29 μM, 0.122 μM and 0.017 μM for BRD2, BRD4, BRD9 and CECR2, respectively.
Targets
CECR2 [1] BRD9 [1] BRD4 [1] BRD2 [1]
17 nM 0.122 μM 0.29 μM 0.41 μM
In vitro
In vitro In cell-based assays, Bromosporine (1 µM) accelerates FRAP recovery of BRD4 and CREBBP, while shows no activities against TIF1α, BAZ2A, and SMARCA2 even at 10 µM. Bromosporine shows moderate cytotoxicity in HeLa cells at 18 µM. Bromosporine, as a chemical probe for bromodomain functional assays, will be very useful in elucidating further biological roles of reader domains. [2]

Chemical Information & Solubility

Molecular Weight 404.44 Formula

C17H20N6O4S
CAS No. 1619994-69-2 SDF Download Bromosporine SDF
Smiles CCOC(=O)NC1=CC(=NN2C1=NN=C2C)C3=CC(=C(C=C3)C)NS(=O)(=O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 42 mg/mL ( (103.84 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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