Home Immunology & Inflammation STING activator - IFN activator 2',3'-cGAMP Sodium Salt For research use only.

2',3'-cGAMP Sodium Salt

Synonyms: 2'-3'-cyclic GMP-AMP Sodium, 2',3'-cGAMP Sodium

2',3'-cGAMP Sodium Salt (2'-3'-cyclic GMP-AMP Sodium) is produced in response to DNA in the cytoplasm in mammalian cells and binds STING with high affinity and is an effective inducer of interferon-β (IFNβ). 2',3'-cGAMP binds to STING with Kd of 3.79 nM.

2',3'-cGAMP Sodium Salt Chemical Structure

2',3'-cGAMP Sodium Salt Chemical Structure

CAS No. 2734858-36-5

Purity & Quality Control

Products often used together with 2',3'-cGAMP Sodium Salt

Vadimezan (DMXAA)

2',3'-cGAMP, and Vadimezan (DMXAA) are both capable of re-educating M2 cells towards an M1 phenotype.

Downey CM, et al. PLoS One. 2014 Jun 18;9(6):e99988.

BV-6

2',3'-cGAMP, and BV6 together exert antitumor effects on PC cells.

Hannes S, et al. Cell Death Dis. 2021 Aug 30;12(9):816.

3',3'-cGAMP

Both 2',3'-cGAMP and 3',3'-cGAMP equally mature human monocyte-derived DCs, upregulating CD40/CD80/CD86/HLA-DR markers.

Gutjahr A, et al. JCI Insight. 2019 Apr 4; 4(7): e125107.

diABZI STING agonist (Compound 3)

2',3'-cGAMP Sodium Salt and diABZI STING agonist demonstrate the most potent, broad-spectrum antiviral function.

Jr GG, et al. Cell Rep Med. 2023 May 16;4(5):101024.

2',3'-cGAMP Sodium Salt Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
293T Function assay 30 mins Activation of recombinant human STING haplotype R71H/G230A/R293Q mutant expressed in 293T cells measured after 30 mins in presence of digitonin A by bright Glo-luciferase reporter gene assay, EC50 = 0.02 μM. 31715099
293T Function assay 30 mins Activation of recombinant human wild-type STING expressed in 293T cells measured after 30 mins in presence of digitonin A by bright Glo-luciferase reporter gene assay, EC50 = 0.02 μM. 31715099
293T Function assay 30 mins Activation of recombinant human STING haplotype G230A/R293Q mutant expressed in 293T cells measured after 30 mins in presence of digitonin A by bright Glo-luciferase reporter gene assay, EC50 = 0.04 μM. 31715099
293T Function assay 30 mins Activation of recombinant human STING haplotype R293Q mutant expressed in 293T cells measured after 30 mins in presence of digitonin A by bright Glo-luciferase reporter gene assay, EC50 = 0.05 μM. 31715099
293T Function assay 30 mins Activation of recombinant human STING haplotype R232H mutant expressed in 293T cells measured after 30 mins in presence of digitonin A by bright Glo-luciferase reporter gene assay, EC50 = 0.07 μM. 31715099
293T Function assay 7 hrs Activation of recombinant human wild-type STING expressed in 293T cells incubated for 7 hrs in absence of digitonin A by bright Glo-luciferase reporter gene assay, EC50 = 13.7 μM. 31715099
THP1 Function assay 20 hrs Agonist activity at STING in human THP1 cells assessed as stimulation of IRF3 pathway measured after 20 hrs by luciferase reporter gene assay, EC50 = 38.6 μM. 31820985
PBMC Function assay 69.6 uM 4 hrs Agonist activity at STING in human PBMC cells assessed as increase in CXCL10 mRNA level at 69.6 uM measured after 4 hrs by qRT-PCR analysis 31820985
PBMC Function assay 1.39 to 139 uM 4 hrs Agonist activity at STING in human PBMC cells assessed as increase in IFNbeta release at 1.39 to 139 uM measured after 4 hrs by ELISA 31820985
PBMC Function assay 69.6 uM 4 hrs Agonist activity at STING in human PBMC cells assessed as increase in IFNbeta mRNA level at 69.6 uM measured after 4 hrs by qRT-PCR analysis 31820985
PBMC Function assay 69.6 uM 4 hrs Agonist activity at STING in human PBMC cells assessed as increase in IL6 mRNA level at 69.6 uM measured after 4 hrs by qRT-PCR analysis 31820985
PBMC Function assay 139 uM 4 hrs Agonist activity at STING in human PBMC cells assessed as increase in IL6 production at 139 uM measured after 4 hrs by ELISA 31820985
B16-OVA Antitumor assay Antitumor activity against mouse B16-OVA cells implanted in mouse assessed as tumour regression in injected flank at 10 ug administered intratumorally on day 6, 9 and 12 post implantation 31500996
B16-OVA Antitumor assay Antitumor activity against mouse B16-OVA cells implanted in mouse assessed as tumour regression in contralateral flank at 10 ug administered intratumorally on day 6, 9 and 12 post implantation 31500996
B16-OVA Antitumor assay Antitumor activity against mouse B16-OVA cells implanted in mouse assessed as higher number of mouse cured of tumors at 10 ug administered intratumorally on day 6, 9 and 12 post implantation 31500996
Click to View More Cell Line Experimental Data

Biological Activity

Description 2',3'-cGAMP Sodium Salt (2'-3'-cyclic GMP-AMP Sodium) is produced in response to DNA in the cytoplasm in mammalian cells and binds STING with high affinity and is an effective inducer of interferon-β (IFNβ). 2',3'-cGAMP binds to STING with Kd of 3.79 nM.
Targets
STING [1]
(Cell-free assay)
3.79 nM(Kd)
In vitro
In vitro

2',3'-cGAMP is an endogenous second messenger produced by mammalian cells. 2',3'-cGAMP is a high affinity ligand for STING. 2',3'-cGAMP is a potent inducer of type-I interferons. 2',3'-cGAMP binding induces conformational changes of STING.[1]
Cell Research Cell lines A549, BSC-40, BSR-T7, Vero cells
Concentrations 25 μM, 20 μM, 2.5 μM
Incubation Time 30 min
Method

A549 cells are infected at an MOI of 5 with WT VACV or VACV-ΔPoxin and lysed 5 hpi. As a positive control, cells are permeabilized for 30 min with digitonin buffer (10 μg ml−1 digitonin, 50 mM HEPES-KOH pH 7.5, 100 mM KCl, 3 mM MgCl2, 0.1 mM DTT, 85 mM sucrose, 0.2% BSA, 1 mM ATP, and 0.1 mM GTP) and stimulated with or without 25 μM 2',3'-cGAMP. After stimulation, the cells are washed once with DMEM supplemented with 10% FBS, media is replaced, and cells are incubated 5 h before lysis.

Chemical Information & Solubility

Molecular Weight 718.37 Formula

C20H22N10Na2O13P2
CAS No. 2734858-36-5 SDF --
Density g/mL
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (139.2 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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