2',3'-cGAMP Sodium Salt

Catalog No.S7904 Batch:S790401

Technical Data

Formula	C₂₀H₂₂N₁₀Na₂O₁₃P₂
Molecular Weight	718.37	CAS No.	2734858-36-5
Solubility (25°C)*	In vitro	Water	50 mg/mL (69.6 mM)
		DMSO	12.5 mg/mL (17.4 mM)

* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

2',3'-cGAMP Sodium Salt (2'-3'-cyclic GMP-AMP Sodium) is produced in response to DNA in the cytoplasm in mammalian cells and binds STING with high affinity and is an effective inducer of interferon-β (IFNβ). 2',3'-cGAMP binds to STING with Kd of 3.79 nM.

Targets

STING ^[1]
(Cell-free assay)

3.79 nM(Kd)

In vitro

2',3'-cGAMP is an endogenous second messenger produced by mammalian cells. 2',3'-cGAMP is a high affinity ligand for STING. 2',3'-cGAMP is a potent inducer of type-I interferons. 2',3'-cGAMP binding induces conformational changes of STING.^[1]

Density

g/mL

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines A549, BSC-40, BSR-T7, Vero cells Concentrations 25 μM, 20 μM, 2.5 μM Incubation Time 30 min Method A549 cells are infected at an MOI of 5 with WT VACV or VACV-ΔPoxin and lysed 5 hpi. As a positive control, cells are permeabilized for 30 min with digitonin buffer (10 μg ml−1 digitonin, 50 mM HEPES-KOH pH 7.5, 100 mM KCl, 3 mM MgCl2, 0.1 mM DTT, 85 mM sucrose, 0.2% BSA, 1 mM ATP, and 0.1 mM GTP) and stimulated with or without 25 μM 2',3'-cGAMP. After stimulation, the cells are washed once with DMEM supplemented with 10% FBS, media is replaced, and cells are incubated 5 h before lysis.

Cell Assay:^{^[2]}

Cell lines
A549, BSC-40, BSR-T7, Vero cells
Concentrations
25 μM, 20 μM, 2.5 μM
Incubation Time
30 min
Method
A549 cells are infected at an MOI of 5 with WT VACV or VACV-ΔPoxin and lysed 5 hpi. As a positive control, cells are permeabilized for 30 min with digitonin buffer (10 μg ml−1 digitonin, 50 mM HEPES-KOH pH 7.5, 100 mM KCl, 3 mM MgCl2, 0.1 mM DTT, 85 mM sucrose, 0.2% BSA, 1 mM ATP, and 0.1 mM GTP) and stimulated with or without 25 μM 2',3'-cGAMP. After stimulation, the cells are washed once with DMEM supplemented with 10% FBS, media is replaced, and cells are incubated 5 h before lysis.

References

Selleck's 2',3'-cGAMP Sodium Salt has been cited by 2 publications

mTORC2-driven chromatin cGAS mediates chemoresistance through epigenetic reprogramming in colorectal cancer [ Nat Cell Biol, 2024, 10.1038/s41556-024-01473-0]	PubMed: 39080411
Novel chemotype NLRP3 inhibitors that target the CRID3-binding pocket with high potency [ Life Sci Alliance, 2024, 7(6)e202402644]	PubMed: 38519142

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.