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CCCP

Name: CCCP
Brand: Selleck Chemicals
SKU: S6494
Rating: 5 (24 reviews)

Synonyms: Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

Catalog No.S6494 Purity:99.71%

CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).

CCCP Chemical Structure

CAS No. 555-60-2

Purity & Quality Control

Batch: Purity: 99.71%

99.71

CCCP Related Products

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Related Compound Libraries	Kinase Inhibitor Library FDA-approved Drug Library Natural Product Library Bioactive Compound Library-I Bioactive Compound Library-Ⅱ	Click to Expand

Biological Activity

Description

In vitro
In vitro	CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.^[1]
Cell Research	Cell lines	cells freshly isolated from patient material or PDXs (patient-derived xenografts)
	Concentrations	25 µM
	Incubation Time	4 h
	Method	5× 10⁵ tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.

In Vivo
In vivo	Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), an oxidative phosphorylation (OXPHOS) uncoupler, kills bacterial cells and prevents forming of biofilm.
Animal Research	Animal Models	Female Balb/c mice
	Dosages	3 mg/kg
	Administration	i.v.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05267808	Recruiting	Obstructive Sleep Apnea	University Hospital Antwerp\|Universiteit Antwerpen	February 1 2022	--

References

Chemical Information & Solubility

Molecular Weight	204.62	Formula	C₉H₅ClN₄
CAS No.	555-60-2	SDF	--
Smiles	C1=CC(=CC(=C1)Cl)NN=C(C#N)C#N
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 41 mg/mL ( (200.37 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 14 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5% DMSO 1 95% Corn oil	2.0mg/ml (9.77mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.0mg/ml (9.77mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 Corn oil	3.0mg/ml (14.66mM)	Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	3.0mg/ml (14.66mM)

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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