CCCP

Catalog No.S6494 Batch:S649401

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Technical Data

Formula

C9H5ClN4

Molecular Weight 204.62 CAS No. 555-60-2
Solubility (25°C)* In vitro DMSO 41 mg/mL (200.37 mM)
Ethanol 21 mg/mL (102.62 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, can inhibits STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP).
In vitro

CCCP, a drug perturbing adenosine triphosphate synthesis by transporting protons across the mitochondrial inner membrane, reduces the spheroid-forming cell (SFC) frequency, indicating sensitivity of stem-like cells towards oxidative phosphorylation (OXPHOS) inhibition.[1]

In vivo

Carbonyl Cyanide 3-Chlorophenylhydrazone (CCCP), an oxidative phosphorylation (OXPHOS) uncoupler, kills bacterial cells and prevents forming of biofilm.

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    cells freshly isolated from patient material or PDXs (patient-derived xenografts)

  • Concentrations

    25 µM

  • Incubation Time

    4 h

  • Method

    5× 105 tumor spheroid cells are seeded into two wells of 6-well ultra-low attachment plates. After seven days, 25 µM CCCP or DMSO are added and cells are incubated for 4 h at 37 ℃. Cells are dissociated, stained with 200 nM TOTO-3 in PBS, and prepared for cell sorting as described. Living cells are sorted into 96-well ultra-low attachment plates containing 100 µL of fresh culture medium supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin per well. Limiting dilution and determination of SFC frequency are performed.

Animal Study:

[2]

  • Animal Models

    Female Balb/c mice

  • Dosages

    3 mg/kg

  • Administration

    i.v.

Selleck's CCCP has been cited by 27 publications

TBC1D23 mediates Golgi-specific LKB1 signaling [ Nat Commun, 2024, 15(1):1785] PubMed: 38413626
Gonococcal OMV-delivered PorB induces epithelial cell mitophagy [ Nat Commun, 2024, 15(1):1669] PubMed: 38396029
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis [ Redox Biol, 2024, 69:103002] PubMed: 38142583
Fut2 Deficiency Promotes Intestinal Stem Cell Aging by Damaging Mitochondrial Functions via Down-Regulating α1,2-Fucosylation of Asah2 and Npc1 [ Research (Wash D C), 2024, 7:0343] PubMed: 38550777
Rictor/mTORC2 signalling contributes to renal vascular endothelial-to-mesenchymal transition and renal allograft interstitial fibrosis by regulating BNIP3-mediated mitophagy [ Clin Transl Med, 2024, 14(5):e1686] PubMed: 38769658
Alpha-lipoic acid alleviates cognitive deficits in transgenic APP23/PS45 mice through a mitophagy-mediated increase in ADAM10 α-secretase cleavage of APP [ Alzheimers Res Ther, 2024, 16(1):160] PubMed: 39030577
PDGF-BB accelerates TSCC via fibroblast lactates limiting miR-26a-5p and boosting mitophagy [ Cancer Cell Int, 2024, 24(1):5] PubMed: 38169376
Mitochondrial elongation confers protection against doxorubicin-induced cardiotoxicity [ Biochem Pharmacol, 2024, 229:116495] PubMed: 39159875
Thiostrepton as a Potential Therapeutic Agent for Hepatocellular Carcinoma [ Int J Mol Sci, 2024, 25(17)9717] PubMed: 39273665
Exogenous NADH promotes the bactericidal effect of aminoglycoside antibiotics against Edwardsiella tarda [ Virulence, 2024, 15(1):2367647] PubMed: 38884466

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.