Home NF-κB IκB/IKK modulator WS3 For research use only.

WS3

WS3 is a β cell proliferation inducer via modulation of Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.

WS3 Chemical Structure

WS3 Chemical Structure

CAS No. 1421227-52-2

Purity & Quality Control

Batch: S744101 DMSO]100 mg/mL]false]Ethanol]80 mg/mL]false]Water]Insoluble]false Purity: 99.04%
99.04

WS3 Related Products

Signaling Pathway

IκB/IKK Signaling Pathways

IκB/IKK Signaling Pathway

Biological Activity

Description WS3 is a β cell proliferation inducer via modulation of Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.
Targets
EBP1 [1] IκB kinase [1]
In vitro
In vitro WS3 induces proliferation of the mouse β cell line R7T1 with EC50 of 0.074 μM. [1] WS3 reversibly induces proliferation of primary fetal and adult human retinal pigment epithelial (RPE) cells. [2]
Cell Research Cell lines R7T1 cells
Concentrations ~10 μM
Incubation Time 4 days
Method R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells.

Chemical Information & Solubility

Molecular Weight 569.58 Formula

C28H30F3N7O3
CAS No. 1421227-52-2 SDF Download WS3 SDF
Smiles CN1CCN(CC1)CC2=C(C=C(C=C2)NC(=O)NC3=CC=C(C=C3)OC4=NC=NC(=C4)NC(=O)C5CC5)C(F)(F)F
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (175.56 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 80 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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