Home NF-κB IκB/IKK modulator WS6 For research use only.

WS6

WS6 is a β cell proliferation inducer via modulation of Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.

WS6 Chemical Structure

WS6 Chemical Structure

CAS No. 1421227-53-3

Purity & Quality Control

WS6 Related Products

Signaling Pathway

IκB/IKK Signaling Pathways

IκB/IKK Signaling Pathway

Biological Activity

Description WS6 is a β cell proliferation inducer via modulation of Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.
Targets
EBP1 [1] IκB kinase [1]
In vitro
In vitro WS6 induces R7T1 cell proliferation with EC50 of 0.28 μM, and promotes β cell proliferation in rodent and human primary islets. Both Erb3 binding protein-1 and the IκB kinase pathway are confirmed to function in the mechanism of action of WS6. [1]
Cell Research Cell lines R7T1 cells
Concentrations ~10 μM
Incubation Time 4 days
Method R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells.
In Vivo
In vivo In RIP-DTA mouse model of β cell ablation, WS6 (50 mg/kg p.o.) ameliorates diabetes, and induces concomitant increases in β cell proliferation and β cell number. [1]

Chemical Information & Solubility

Molecular Weight 568.59 Formula

C29H31F3N6O3
CAS No. 1421227-53-3 SDF Download WS6 SDF
Smiles CN1CCN(CC1)CC2=C(C=C(C=C2)NC(=O)CC3=CC=C(C=C3)OC4=NC=NC(=C4)NC(=O)C5CC5)C(F)(F)F
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (175.87 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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