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Vitamin C

Synonyms: L-Ascorbic acid

Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.

Vitamin C Chemical Structure

Vitamin C Chemical Structure

CAS No. 50-81-7

Purity & Quality Control

Vitamin C Related Products

Biological Activity

Description Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.
In vitro
In vitro

Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. [1]

Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. [2]

Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells. [3]

Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. [4]
Cell Research Cell lines Fibroblasts
Concentrations 100 μM
Incubation Time 2 h
Method

Cells were treated with indicated concentration of drug for 2 h.
In Vivo
In vivo

Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06206759 Not yet recruiting
Hypertriglyceridemia
Meir Medical Center
 May 1 2024 --
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Dietary Exposure|Diabetes Mellitus Type 2|Cardiovascular Diseases|Bone Loss|Sustainability|Exposure|Obesity|Vitamin Deficiency|Mineral Deficiency
German Federal Institute for Risk Assessment|Max Rubner-Institut|University of Bonn|Research Institute for Plant-Based Nutrition|University of Jena|University of Regensburg|Heidelberg University|University of Vienna
 April 1 2024 --
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Healthy
University of East Anglia|The Norfolk and Norwich University Hospitals NHS Foundation Trust|The Quadram Institute Clinical Research Facility
 February 2024 Not Applicable
NCT06244758 Recruiting
Type2diabetes
University of Erlangen-Nürnberg Medical School|Bayer
 January 18 2024 Phase 3

Chemical Information & Solubility

Molecular Weight 176.12 Formula

C6H8O6
CAS No. 50-81-7 SDF Download Vitamin C SDF
Smiles C(C(C1C(=C(C(=O)O1)O)O)O)O
Storage (From the date of receipt) 3 years -20°C powder (seal)

In vitro
Batch:

DMSO : 35 mg/mL ( (198.72 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 35 mg/mL

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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