Vitamin C

Catalog No.S3114 Batch:S311402

Technical Data

Formula	C₆H₈O₆
Molecular Weight	176.12	CAS No.	50-81-7
Solubility (25°C)*	In vitro	DMSO	35 mg/mL (198.72 mM)
		Water	35 mg/mL (198.72 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.
In vitro	Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. ^[1] Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. ^[2] Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells. ^[3] Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. ^[4]
In vivo	Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. ^[5]

Description

Vitamin C (L-Ascorbic acid) is a water-soluble vitamin indicated for the prevention and treatment of scurvy.

In vitro

Ascorbate when combined with copper rapidly reduces the viscosity of DNA solutions and has exhibited some carcinostatic effects on transplanted sarcoma 180 tumours in mice. ^[1]

Exposure of HL-60 cells to increasing concentrations of H2O2 results in dose-dependent intracellular accumulation of peroxides, which is accompanied by a decrease in intracellular ascorbic acid and an increase in apoptosis. Ascorbic acid loading also provides strong protection from apoptosis associated with serum- and glucose-free culture of HL-60 cells. ^[2]

Vitamin C is found to protect GSH-depleted HL-60 cells in a dose-dependent manner. Vitamin C inhibits the formation of ROS in both normal and GSH-depleted cells. ^[3]

Ascorbate (1 mM) generates 161 mM H2O2 in Dulbecco's modified Eagle's medium and induces apoptosis in 50% of HL60 cells, whereas in RPMI 1640 only 83 mM H2O2 is produced and no apoptosis is detected. ^[4]

In vivo

Treatment of rats with Vitamin C 10 min prior to the cisplatin inhibits cisplatin-mediated damage. The enhanced serum creatinine levels which are indicative of renal injury shows a significant reduction with the three doses of Vitamin C tested. ^[5]

Protocol (from reference)

Cell Assay:^{^[6]}	Cell lines Fibroblasts Concentrations 100 μM Incubation Time 2 h Method Cells were treated with indicated concentration of drug for 2 h.

References

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Selleck's Vitamin C has been cited by 17 publications

Long noncoding RNA Malat1 protects against osteoporosis and bone metastasis [ Nat Commun, 2024, 15(1):2384]	PubMed: 38493144
DNA Methylation-Mediated GPX4 Transcriptional Repression and Osteoblast Ferroptosis Promote Titanium Particle-Induced Osteolysis [ Research (Wash D C), 2024, 7:0457]	PubMed: 39161535
RNA m6A modification regulates L1 retrotransposons in human spermatogonial stem cell differentiation in vitro and in vivo [ Cell Mol Life Sci, 2024, 81(1):92]	PubMed: 38363375
Conversion of glioma cells into neuron-like cells by small molecules [ iScience, 2024, 27(11):111091]	PubMed: 39483145
Loss of SHROOM3 affects neuroepithelial cell shape through regulating cytoskeleton proteins in cynomolgus monkey organoids [ Zool Res, 2024, 45(2):233-241]	PubMed: 38287904
Combination of Vitamin C and Lenvatinib potentiates antitumor effects in hepatocellular carcinoma cells in vitro [ PeerJ, 2023, 11:e14610]	PubMed: 36718449
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1–mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644]	PubMed: 36993303
Long noncoding RNA Malat1 inhibits Tead3-Nfatc1-mediated osteoclastogenesis to suppress osteoporosis and bone metastasis [ Res Sq, 2023, rs.3.rs-2405644]	PubMed: 36993303
An organoid model of colorectal circulating tumor cells with stem cell features, hybrid EMT state and distinctive therapy response profile [ J Exp Clin Cancer Res, 2022, 41(1):86]	PubMed: 35260172
Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer Cells [ Metabolites, 2022, 12(3)267]	PubMed: 35323710

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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