Home Metabolism Vitamin chemical Trolox For research use only.

Trolox

Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.

Trolox Chemical Structure

Trolox Chemical Structure

CAS No. 53188-07-1

Purity & Quality Control

Trolox Related Products

Biological Activity

Description Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.
In vitro
In vitro Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-Carboxylic Acid) is a vitamin E analog and a direct scavenger of peroxyl and alkoxyl radicals. Trolox impairs PT4 cell survival, has minor effects on ROS levels and causes wide deregulation of cell cycle genes[1]. Trolox enhances As2O3-mediated apoptosis in APL, myeloma, and breast cancer cells. Trolox inhibits DNA damage formation induced by singlet oxygen in human lymphoblast WTK-1 cells and protects red blood cells during photodynamic treatment[2].
Cell Research Cell lines NB4, IM9, and AsR2 cells
Concentrations 100 μM
Incubation Time 6 days
Method NB4, IM9, and AsR2 cells are seeded at 1 × 105 cells/mL in 24-well plates. Cells are treated with various concentrations of As2O3 or doxorubicin, alone or in combination with 100 μM trolox for 6 days. Viable cells are counted by trypan blue exclusion on days 1, 3, and 6. All cells are maintained at a density lower than 1 × 106 cells/mL through dilution as required, and media ± treatment is replaced every third day. MCF-7, MDA-231, and T47D are seeded in 24-well plates at a density of 4000 cells/well. The next day, fresh media containing As2O3 ± trolox is added. On the days indicated, cells are fixed in 10% trichloroacetic acid and subsequently stained with sulforhodamine B (SRB). Bound SRB is solubilized in 10 mM unbuffered Tris (tris(hydroxymethyl)aminomethane), and optical density is measured at 570 nm in a microplate reader.
In Vivo
In vivo After cerebral ischemia reperfusion, Trolox prescription increases anti-apoptotic proteins and decreased proapoptotic proteins thus protects neurons of the hippocampus and caused improvement of memory[3].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05937737 Completed
Type 2 Diabetes
Hacettepe University
 April 20 2020 Not Applicable
NCT04716764 Unknown status
Breast Cancer|Female
Necmettin Erbakan University|Hacettepe University|Turkish Society of Medical Oncology
 March 3 2020 --
NCT04053569 Unknown status
Metabolic Syndrome Protection Against
Azienda Ospedaliera Specializzata in Gastroenterologia Saverio de Bellis
 October 1 2019 Not Applicable

Chemical Information & Solubility

Molecular Weight 250.29 Formula

C14H18O4
CAS No. 53188-07-1 SDF Download Trolox SDF
Smiles CC1=C(C2=C(CCC(O2)(C)C(=O)O)C(=C1O)C)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 50 mg/mL ( (199.76 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 50 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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