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Thimerosal

Synonyms: Thiomersalate, Mercurothiolate

Thimerosal (Thiomersalate, Mercurothiolate) is a well-established antiseptic and antifungal agent and usually used as a preservative in vaccines, immunoglobulin preparations, skin test antigens, antivenins, ophthalmic and nasal products, and tattoo inks. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

Thimerosal Chemical Structure

Thimerosal Chemical Structure

CAS No. 54-64-8

Purity & Quality Control

Batch: S364601 DMSO]80 mg/mL]false]Water]80 mg/mL]false]Ethanol]20 mg/mL]false Purity: 98.31%
98.31

Thimerosal Related Products

Biological Activity

Description Thimerosal (Thiomersalate, Mercurothiolate) is a well-established antiseptic and antifungal agent and usually used as a preservative in vaccines, immunoglobulin preparations, skin test antigens, antivenins, ophthalmic and nasal products, and tattoo inks. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
In vitro
In vitro The treatment of Jurkat T cells with thimerosal causes a significant decrease in cellular viability at 1 μM (25%, p<0.05; IC50: 10 μM)[1]. Thimerosal is also able to induce apoptosis and G2/M phase in human leukemia U937 cells[2].
Cell Research Cell lines Jurkat T cells
Concentrations 0.01, 0.1, 1, 10, 50, 100, 250 μM
Incubation Time 48 h
Method The number of 1×104 cells/well Jurkat T cells are seeded into 96 well plates and exposed to thimerosal, methyl mercury, and mercuric chloride (HgCl2) at concentrations of thimerosal (0.01-0.1-1-10-50-100-250 μM), methyl mercury (30-50-80-100-250 μM), and mercuric chloride (HgCl2 (20-40-60-80-100 μM). The plates are incubated at 37°C in a humidified atmosphere containing 5% CO2. After 48 hours, the medium is discarded and 20 μl/well of MTT solution (5 mg/ml) is added and incubated for 3 hours at 37°C (5% CO2). Finally, 20μl/well of isopropanol is added and the color intensity is read spectrophotometrically at 590 nm.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00795951 Completed
Dermatitis Contact
Allerderm
 November 2008 Phase 4

Chemical Information & Solubility

Molecular Weight 404.81 Formula

C9H9HgO2S.Na
CAS No. 54-64-8 SDF Download Thimerosal SDF
Smiles CC[Hg]SC1=CC=CC=C1C(=O)[O-].[Na+]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 80 mg/mL ( (197.62 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 80 mg/mL

Ethanol : 20 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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