Thimerosal

Catalog No.S3646 Batch:S364601

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Technical Data

Formula

C9H9HgO2S.Na
Molecular Weight 404.81 CAS No. 54-64-8
Solubility (25°C)* In vitro DMSO 80 mg/mL (197.62 mM)
Water 80 mg/mL (197.62 mM)
Ethanol 20 mg/mL (49.4 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Thimerosal (Thiomersalate, Mercurothiolate) is a well-established antiseptic and antifungal agent and usually used as a preservative in vaccines, immunoglobulin preparations, skin test antigens, antivenins, ophthalmic and nasal products, and tattoo inks. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
In vitro The treatment of Jurkat T cells with thimerosal causes a significant decrease in cellular viability at 1 μM (25%, p<0.05; IC50: 10 μM)[1]. Thimerosal is also able to induce apoptosis and G2/M phase in human leukemia U937 cells[2].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    Jurkat T cells

  • Concentrations

    0.01, 0.1, 1, 10, 50, 100, 250 μM

  • Incubation Time

    48 h

  • Method

    The number of 1×104 cells/well Jurkat T cells are seeded into 96 well plates and exposed to thimerosal, methyl mercury, and mercuric chloride (HgCl2) at concentrations of thimerosal (0.01-0.1-1-10-50-100-250 μM), methyl mercury (30-50-80-100-250 μM), and mercuric chloride (HgCl2 (20-40-60-80-100 μM). The plates are incubated at 37°C in a humidified atmosphere containing 5% CO2. After 48 hours, the medium is discarded and 20 μl/well of MTT solution (5 mg/ml) is added and incubated for 3 hours at 37°C (5% CO2). Finally, 20μl/well of isopropanol is added and the color intensity is read spectrophotometrically at 590 nm.

Selleck's Thimerosal has been cited by 3 publications

Cancer cell-intrinsic biosynthesis of itaconate promotes tumor immunogenicity [ EMBO J, 2024, 10.1038/s44318-024-00217-y] PubMed: 39349845
Romidepsin and afatinib abrogate JAK-STAT signaling and elicit synergistic antitumor effects in cutaneous T-cell lymphoma [ J Invest Dermatol, 2024, S0022-202X(23)03210-4] PubMed: 38219917
An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunity [ Cell Chem Biol, 2021, S2451-9456(21)00442-6] PubMed: 34672954

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.