Home Endocrinology & Hormones Androgen Receptor antagonist Spironolactone For research use only.

Spironolactone

Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.

Spironolactone Chemical Structure

Spironolactone Chemical Structure

CAS No. 52-01-7

Purity & Quality Control

Spironolactone Related Products

Signaling Pathway

Androgen Receptor Signaling Pathways

Androgen Receptor Signaling Pathway

Biological Activity

Description Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.
Targets
Androgen Receptor [1]
77 nM
In vitro
In vitro Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). [1] Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. [2]
In Vivo
In vivo Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. [2]
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Chemical Information & Solubility

Molecular Weight 416.57 Formula

C24H32O4S
CAS No. 52-01-7 SDF Download Spironolactone SDF
Smiles CC(=O)SC1CC2=CC(=O)CCC2(C3C1C4CCC5(C4(CC3)C)CCC(=O)O5)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 83 mg/mL ( (199.24 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 27 mg/mL

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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