Spironolactone

Catalog No.S4054 Batch:S405402

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Technical Data

Formula

C24H32O4S

Molecular Weight 416.57 CAS No. 52-01-7
Solubility (25°C)* In vitro DMSO 83 mg/mL (199.24 mM)
Ethanol 20 mg/mL (48.01 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM.
Targets
Androgen Receptor [1]
77 nM
In vitro Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). [1] Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. [2]
In vivo Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. [2]

Protocol (from reference)

Customer Product Validation

Data from [Data independently produced by , , Leukemia, 2018, 32(1):111-119]

Data from [Data independently produced by , , Antiviral Res, 2017, 146:76-85]

Selleck's Spironolactone has been cited by 13 publications

Profiling of ERBB receptors and downstream pathways reveals selectivity and hidden properties of ERBB4 antagonists [ iScience, 2024, 27(2):108839] PubMed: 38303712
An Integrated Approach of Bioassays and Non-Target Screening for the Assessment of Endocrine-Disrupting Activities in Tap Water and Identification of Novel Endocrine-Disrupting Chemicals [ Toxics, 2024, 12(4)247] PubMed: 38668470
LP-284, a small molecule acylfulvene, exerts potent antitumor activity in preclinical non-Hodgkin's lymphoma models and in cells deficient in DNA damage repair [ Oncotarget, 2023, 14:597-611] PubMed: 37306526
NR3C2 inhibits the proliferation of colorectal cancer via regulating glucose metabolism and phosphorylating AMPK [ J Cell Mol Med, 2023, 27(8):1069-1082] PubMed: 36950803
Direct actions of dapagliflozin and interactions with LCZ696 and spironolactone on cardiac fibroblasts of patients with heart failure and reduced ejection fraction [ ESC Heart Fail, 2022, 10.1002/ehf2.14186] PubMed: 36303443
Inhibitors of Nucleotide Excision Repair Decrease UVB-Induced Mutagenesis-An In Vitro Study [ Int J Mol Sci, 2021, 22(4)1638] PubMed: 33562002
Pannexin 1 binds β-catenin to modulate melanoma cell growth and metabolism [ J Biol Chem, 2021, S0021-9258(21)00252-0] PubMed: 33647315
In vitro Assessment of Corticosteroid Effects of Eight Chiral Herbicides [ J Environ Sci Health B, 2020, 55(2):91-102] PubMed: 31524045
Nucleotide excision repair is a potential therapeutic target in multiple myeloma [Szalat R, et al. Leukemia, 2018, 32(1):111-119] PubMed: 28588253
Potential endocrine-disrupting effects of metals via interference with glucocorticoid and mineralocorticoid receptors. [ Environ Pollut, 2018, 242(Pt A):12-18] PubMed: 29957541

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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