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Technical Data
| Formula | C24H32O4S |
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| Molecular Weight | 416.57 | CAS No. | 52-01-7 | |
| Solubility (25°C)* | In vitro | DMSO | 83 mg/mL (199.24 mM) | |
| Ethanol | 27 mg/mL (64.81 mM) | |||
| Water | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Spironolactone is a potent antagonist of the androgen receptor with IC50 of 77 nM. | ||
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| Targets |
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| In vitro | Spironolactone is a strong AR antagonist (IC50 ~ 77 nM), a weak GR antagonist (IC50 ~ 2.4 μM), and a weak PR agonist (EC50 ~ 740 nM). [1] Spironolactone inhibits androstanolone binding in rat prostate nuclei and androstanolone specific binding in rat prostate cytosol. [2] | ||
| In vivo | Spironolactone (1 mg/day) exerts anti-androgenic activity in rats. A single pretreatment of spironolactone (1 mg/rat) inhibits specific and saturable uptake of hormone into prostate induced by tracer dose administration of [3H]testosterone. [2] |
Protocol (from reference)
References
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Customer Product Validation
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Data from [Data independently produced by , , Leukemia, 2018, 32(1):111-119]
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Data from [Data independently produced by , , Antiviral Res, 2017, 146:76-85]
Selleck's Spironolactone has been cited by 14 publications
| Polypharmacological profiling across protein target families and cellular pathways using the multiplexed cell-based assay platform safetyProfiler reveals efficacy, potency and side effects of drugs [ Biomed Pharmacother, 2024, 180:117523] | PubMed: 39405910 |
| Profiling of ERBB receptors and downstream pathways reveals selectivity and hidden properties of ERBB4 antagonists [ iScience, 2024, 27(2):108839] | PubMed: 38303712 |
| An Integrated Approach of Bioassays and Non-Target Screening for the Assessment of Endocrine-Disrupting Activities in Tap Water and Identification of Novel Endocrine-Disrupting Chemicals [ Toxics, 2024, 12(4)247] | PubMed: 38668470 |
| LP-284, a small molecule acylfulvene, exerts potent antitumor activity in preclinical non-Hodgkin's lymphoma models and in cells deficient in DNA damage repair [ Oncotarget, 2023, 14:597-611] | PubMed: 37306526 |
| NR3C2 inhibits the proliferation of colorectal cancer via regulating glucose metabolism and phosphorylating AMPK [ J Cell Mol Med, 2023, 27(8):1069-1082] | PubMed: 36950803 |
| Direct actions of dapagliflozin and interactions with LCZ696 and spironolactone on cardiac fibroblasts of patients with heart failure and reduced ejection fraction [ ESC Heart Fail, 2022, 10.1002/ehf2.14186] | PubMed: 36303443 |
| Inhibitors of Nucleotide Excision Repair Decrease UVB-Induced Mutagenesis-An In Vitro Study [ Int J Mol Sci, 2021, 22(4)1638] | PubMed: 33562002 |
| Pannexin 1 binds β-catenin to modulate melanoma cell growth and metabolism [ J Biol Chem, 2021, S0021-9258(21)00252-0] | PubMed: 33647315 |
| In vitro Assessment of Corticosteroid Effects of Eight Chiral Herbicides [ J Environ Sci Health B, 2020, 55(2):91-102] | PubMed: 31524045 |
| Nucleotide excision repair is a potential therapeutic target in multiple myeloma [Szalat R, et al. Leukemia, 2018, 32(1):111-119] | PubMed: 28588253 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.