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Rituximab (anti-CD20)

Synonyms: IDEC-C2B8

Catalog No.A2009 Purity:99%

Rituximab (anti-CD20) is a chimeric anti-CD20 mAb that binds the CD20 antigen on B cells with a binding affinity of 5 nM, MW: 143.86 KD.

Rituximab (anti-CD20) Chemical Structure

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Selleck's Rituximab (anti-CD20) has been cited by 14 publications

Purity & Quality Control

Batch: Purity: 99% Protein concentration: 19.96mg/ml Endotoxin Level: ＜1EU/mg

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1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation. 2. "✔" indicates inhibitory effect, but without specific value.

Biological Activity

Description

Rituximab (anti-CD20) is a chimeric anti-CD20 mAb that binds the CD20 antigen on B cells with a binding affinity of 5 nM, MW: 143.86 KD.

Features

Human IgG1

Targets

CD20 ^[1]

In Vitro
In vitro	Anti-CD20 IgG1 antibody (rituximab), induces strong antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) effects on CD20+ Raji cells with the EC50 at 0.0365 nM and 1.5 nM, respectively.^[1]
Cell Research:	For ADCC experiments, PBMCs were isolated from healthy human donor blood to serve as effector cells and were plated with target Raji cells at E:T ratio of 50:1 in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Target Raji cells were labeled with calcein-AM for detection of cell lysis prior to experimental set up. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed. For CDC experiments, human complement was plated with calcein-AM labeled target Raji cells in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed.

In Vitro

In vitro

Anti-CD20 IgG1 antibody (rituximab), induces strong antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) effects on CD20+ Raji cells with the EC50 at 0.0365 nM and 1.5 nM, respectively.^[1]

Cell Research:

For ADCC experiments, PBMCs were isolated from healthy human donor blood to serve as effector cells and were plated with target Raji cells at E:T ratio of 50:1 in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Target Raji cells were labeled with calcein-AM for detection of cell lysis prior to experimental set up. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed. For CDC experiments, human complement was plated with calcein-AM labeled target Raji cells in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Following incubation for 4 hours at 37°C in 5% CO₂, supernatants from the experiment were analyzed.

In Vivo
In vivo	A number of in vivo tumor models suggest the anti-tumor activity of rituximab is dependent, at least in part, on complement^[2]. Rituximab can deplete B cells for several months and, as such, could represent an effective therapy for B cell-mediated autoimmune diseases. Rituximab is now widely used in onco-haematology and is currently in development in several autoimmune diseases^[3].
Animal Research	Objective: To study the mechanisms of follicular lymphoma’s resistance to rituximab Animal Models: Female CB17 severe combined immunodeficient(SCID) mice were injected (s.c.) with rituximab-resistant lymphoma cells (derived from a human FL) Formulation: -- Dosages: 10mg/kg, weekly Administration: i.p. Reference: https://www.ncbi.nlm.nih.gov/pubmed/19188155 Objective: To investigate the therapeutic effect of rituximab in combination with L19-IL2 on B-cell malignancies Animal Models: CB17/lcr severe combined mmunodeficiency (SCID) mice were injected (s.c.) Ramos or DoHH-2 lymphoma cells Formulation: saline Dosages: 200 μg Administration: i.v. Reference: https://www.ncbi.nlm.nih.gov/pubmed/19005180 Rituximab can apply to immunodeficient mice (eg: SCID mice), lymphoma cell lines and other related assays (Only for Reference)

References
[1] Muralidharan S, et al. J Pharmacol Exp Ther. 2022 Apr;381(1):12-21. [2] George J. Weiner, et al. Semin Hematol. 2010, 47(2): 115-123. [3] Cartron G, et al. Crit Rev Oncol Hematol. 2007, 62(1):43-52.

References

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	174722-31-7
Isotype	Human IgG1
Source	CHO cells
Formulation	100 mM Pro-Ac, 20 mM Arg, pH 5.0
Storage (From the date of receipt)	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Selleck's selected clones are more frequently applied. (data until September 2024)

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Frequently Asked Questions

Question 1:
How to store the antibody?

Answer:
Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.