Rituximab (anti-CD20)

Catalog No.A2009        Batch: A200911

Print

Technical Data

CAS No. 174722-31-7
Formulation 100 mM Pro-Ac, 20 mM Arg, pH 5.0
Isotype Human IgG1
Source CHO cells
Storage
(From the date of receipt)		 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles
Purity 99%
Protein concentration 19.96mg/ml
Endotoxin Level ＜1EU/mg

Biological Activity

Description Rituximab (anti-CD20) is a chimeric anti-CD20 mAb that binds the CD20 antigen on B cells with a binding affinity of 5 nM, MW: 143.86 KD.
Targets
CD20 [1]
In vitro

Anti-CD20 IgG1 antibody (rituximab), induces strong antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) effects on CD20+ Raji cells with the EC50 at 0.0365 nM and 1.5 nM, respectively.[1]
In vivo

A number of in vivo tumor models suggest the anti-tumor activity of rituximab is dependent, at least in part, on complement[2]. Rituximab can deplete B cells for several months and, as such, could represent an effective therapy for B cell-mediated autoimmune diseases. Rituximab is now widely used in onco-haematology and is currently in development in several autoimmune diseases[3].

Protocol (Only for Reference)

Cell Assay:

  • For ADCC experiments, PBMCs were isolated from healthy human donor blood to serve as effector cells and were plated with target Raji cells at E:T ratio of 50:1 in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Target Raji cells were labeled with calcein-AM for detection of cell lysis prior to experimental set up. Following incubation for 4 hours at 37°C in 5% CO2, supernatants from the experiment were analyzed. For CDC experiments, human complement was plated with calcein-AM labeled target Raji cells in the presence of indicated concentrations of KPL-404, anti-CD20, or isotype antibodies. Following incubation for 4 hours at 37°C in 5% CO2, supernatants from the experiment were analyzed.
Animal Study:

  • Objective: To study the mechanisms of follicular lymphoma’s resistance to rituximab
    Animal Models: Female CB17 severe combined immunodeficient(SCID) mice were injected (s.c.) with rituximab-resistant lymphoma cells (derived from a human FL)
    Formulation: --
    Dosages: 10mg/kg, weekly
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19188155

    Objective: To investigate the therapeutic effect of rituximab in combination with L19-IL2 on B-cell malignancies
    Animal Models: CB17/lcr severe combined mmunodeficiency (SCID) mice were injected (s.c.) Ramos or DoHH-2 lymphoma cells
    Formulation: saline
    Dosages: 200 μg
    Administration: i.v.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19005180

    Rituximab can apply to immunodeficient mice (eg: SCID mice), lymphoma cell lines and other related assays (Only for Reference)

Selleck's Rituximab (anti-CD20) has been cited by 16 publications

Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC [ Sci Rep, 2024, 14(1):7938] PubMed: 38575779
TLR9 agonism differentially impacts human NK cell-mediated direct killing and antibody-dependent cell-mediated cytotoxicity [ Sci Rep, 2024, 14(1):14595] PubMed: 38918496
Downhill running does not alter blood C1q availability or complement-dependent cytotoxicity of therapeutic monoclonal antibodies against haematological cancer cell lines in vitro [ Sci Rep, 2024, 14(1):28239] PubMed: 39548231
Noninvasive PET imaging of tumor PD-L1 expression with 64Cu-labeled Durvalumab [ Am J Nucl Med Mol Imaging, 2024, 14(1):31-40] PubMed: 38500749
Noninvasive PET imaging of tumor PD-L1 expression with 64Cu-labeled Durvalumab [ Am J Nucl Med Mol Imaging, 2024, 14(1):31-40] PubMed: 38500749
Daratumumab induces cell-mediated cytotoxicity of primary effusion lymphoma and is active against refractory disease [ Oncoimmunology, 2023, 12(1):2163784] PubMed: 36632565
Daratumumab induces cell-mediated cytotoxicity of primary effusion lymphoma and is active against refractory disease [ Oncoimmunology, 2023, 12(1):2163784] PubMed: 36632565
Development and implementation of natural killer cell simultaneous ADCC and direct killing assay [ Heliyon, 2023, 9(12):e22991] PubMed: 38125417
Rheumatoid factor IgM autoantibodies control IgG homeostasis [ Front Immunol, 2022, 13:1016263] PubMed: 36341420
Beta-Lapachone Attenuates BMSC-Mediated Neuroblastoma Malignant Transformation by Inhibiting Gal-3/Gal-3BP/IL6 Axis [ J Pharmacol Exp Ther, 2022, 381(1):12-21] PubMed: 35078863

FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.