Home MAPK p38 MAPK inhibitor - TGF-beta/Smad inhibitor - Enterovirus inhibitor PD 169316 For research use only.

PD 169316

PD 169316 is a potent, selective and cell-permeable p38 MAP kinase inhibitor with IC50 of 89 nM. PD169316 abrogates signaling initiated by both TGFbeta and Activin A. PD169316 shows antiviral activity against Enterovirus71.

PD 169316 Chemical Structure

PD 169316 Chemical Structure

CAS No. 152121-53-4

Purity & Quality Control

PD 169316 Related Products

Signaling Pathway

p38 MAPK Signaling Pathways

p38 MAPK Signaling Pathway

Biological Activity

Description PD 169316 is a potent, selective and cell-permeable p38 MAP kinase inhibitor with IC50 of 89 nM. PD169316 abrogates signaling initiated by both TGFbeta and Activin A. PD169316 shows antiviral activity against Enterovirus71.
Targets
TGF-β [1] Activin A [1] Enterovirus71 [2] p38 MAPK [1]
(Cell-free assay)
89 nM
In vitro
In vitro

Inhibition of the P38 kinase activity using 0.5 μM PD169316 significantly changes the proliferation rate and extent of apoptosis; the effects of 0.2 μM PD169316 are not significant. The level of P-STAT1 (Tyr701) decreases with 0.5 μM PD169316, which is probably due to P38 inhibition.[1]
Cell Research Cell lines KBU cells
Concentrations 0.2 μM, 0.5 μM
Incubation Time 1 h
Method

KBU cells are exposed to 12 ppm G. pps alone or with 1 h-pre-exposure to P38 inhibitor PD169316 and analyzed for proliferation (MTT), apoptosis (Ann V) and changes in the level of modifications of proteins involved in the SAPK/JNK and P38 signaling pathways.
In Vivo
In vivo

PD169316 (intramuscular injection every day for 14 consecutive days) shows antiviral activity in a suckling mouse model.

Chemical Information & Solubility

Molecular Weight 360.34 Formula

C20H13FN4O2
CAS No. 152121-53-4 SDF --
Smiles C1=CC(=CC=C1C2=NC(=C(N2)C3=CC=NC=C3)C4=CC=C(C=C4)F)[N+](=O)[O-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 14 mg/mL ( (38.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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