PD 169316

Catalog No.S5183 Batch:S518302

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Technical Data

Formula

C20H13FN4O2
Molecular Weight 360.34 CAS No. 152121-53-4
Solubility (25°C)* In vitro DMSO 14 mg/mL (38.85 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
0.7mg/ml Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% corn oil
0.2mg/ml Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description PD 169316 is a potent, selective and cell-permeable p38 MAP kinase inhibitor with IC50 of 89 nM. PD169316 abrogates signaling initiated by both TGFbeta and Activin A. PD169316 shows antiviral activity against Enterovirus71.
Targets
TGF-β [1] Activin A [1] Enterovirus71 [2] p38 MAPK [1]
(Cell-free assay)
89 nM
In vitro

Inhibition of the P38 kinase activity using 0.5 μM PD169316 significantly changes the proliferation rate and extent of apoptosis; the effects of 0.2 μM PD169316 are not significant. The level of P-STAT1 (Tyr701) decreases with 0.5 μM PD169316, which is probably due to P38 inhibition.[1]
In vivo

PD169316 (intramuscular injection every day for 14 consecutive days) shows antiviral activity in a suckling mouse model.

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    KBU cells

  • Concentrations

    0.2 μM, 0.5 μM

  • Incubation Time

    1 h

  • Method

    KBU cells are exposed to 12 ppm G. pps alone or with 1 h-pre-exposure to P38 inhibitor PD169316 and analyzed for proliferation (MTT), apoptosis (Ann V) and changes in the level of modifications of proteins involved in the SAPK/JNK and P38 signaling pathways.

Selleck's PD 169316 has been cited by 1 publication

Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathway [ PLoS One, 2021, 16(5):e0252541] PubMed: 34048499

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.