Home Immunology & Inflammation PD-1/PD-L1 inhibitor BMS-1 For research use only.

BMS-1

Synonyms: PD-1/PD-L1 inhibitor 1

BMS-1 is a small-molecule inhibitor of PD-1/PD-L1 interaction with IC50 of 6 nM.

BMS-1 Chemical Structure

BMS-1 Chemical Structure

CAS No. 1675201-83-8

Purity & Quality Control

BMS-1 Related Products

Biological Activity

Description BMS-1 is a small-molecule inhibitor of PD-1/PD-L1 interaction with IC50 of 6 nM.
Targets
PD-1/PD-L1 interaction [1]
(Cell-free assay)
0.006 μM
In vitro
In vitro

PD-1/PD-L1 inhibitor 1 causes half-reduced cell viability at lower concentrations in triple-negative breast cancer (TNBC), activates ERK in MDA-MB-231 and MCF7, significantly increases IL-8 expression in MDA-MB-231 and HCC1806 when administrated in combination with ERK1/2 inhibitor, also potently downregulates the IL-8 expression to its half amount in MDA-MB-231.[2]
Cell Research Cell lines MDA-MB-231 cells, HCC1937 cells, HCC1806 cells, MCF7 cells
Concentrations --
Incubation Time 72 h
Method

Cell proliferation is determined by the MTS assay. A total of 1-2×103 cells are plated in 100 μl medium in each well of a 96-well plate. After 24 h, PD-1/PD-L1 inhibitor 1 and ERK1/2 inhibitor are diluted to the desired concentrations in test medium (DMEM containing 1% fetal bovine serum) and added as single or as combined treatment in triplicate for 72 h.
In Vivo
In vivo

PD-1/PD-L1 inhibitor 1 combined with Jiedu Sangen Decoction (JSD) remarkably inhibits and reverses epithelial-mesenchymal transition (EMT) via PI3K/AKT signaling pathway.[3]
Animal Research Animal Models Male specific pathogen free BALB/c mice of hepatic metastatic colorectal cancer (CRC) model
Dosages 100 µg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 475.58 Formula

C29H33NO5
CAS No. 1675201-83-8 SDF Download BMS-1 SDF
Smiles CC1=C(C=CC=C1C2=CC=CC=C2)COC3=CC(=C(C(=C3)OC)CN4CCCCC4C(=O)O)OC
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 95 mg/mL ( (199.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 95 mg/mL

Ethanol : 5 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Frequently Asked Questions

Question 1:
I would like to know the difference of S8158, S7912, S7911, and the reason of why the IC50 is different.

Answer:
These three compounds are three different PD-l/PD-L1 inhibitors. They have different structures, characteristics, and probably function through different mechanisms. And this is why the IC50s are different.

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