Home Metabolism Mitochondrial Metabolism inhibitor NL-1 For research use only.

NL-1

The mitochondrial mitoNEET ligand NL-1 is a mitoNEET inhibitor, which induces a concentration-dependent decrease in cell viability with an IC50 of 47.35 μM and 56.26 μM in REH and REH/Ara-C cells respectively. The docking studies using liver mitochondrial suspensions show the IC50 of NL-1 binding for [3H]-rosiglitazone is 0.9 μM and the Ki for NL-1 site 1 and site 2 was 4.78 and 2.77, respectively.

NL-1 Chemical Structure

NL-1 Chemical Structure

CAS No. 188532-26-5

Purity & Quality Control

Batch: S076701 DMSO]67 mg/mL]false]Ethanol]8 mg/mL]false]Water]Insoluble]false Purity: 99.9%
99.9

NL-1 Related Products

Biological Activity

Description The mitochondrial mitoNEET ligand NL-1 is a mitoNEET inhibitor, which induces a concentration-dependent decrease in cell viability with an IC50 of 47.35 μM and 56.26 μM in REH and REH/Ara-C cells respectively. The docking studies using liver mitochondrial suspensions show the IC50 of NL-1 binding for [3H]-rosiglitazone is 0.9 μM and the Ki for NL-1 site 1 and site 2 was 4.78 and 2.77, respectively.
Targets
mitoNEET [1]
(in [3H]-rosiglitazone binding test)		 mitoNEET [2]
(in REH cells viability test)		 mitoNEET [2]
(in REH/Ara-C cells viability test)
0.9 μM 47.35 μM 56.26 μM
In vitro
In vitro

NL-1 is a mitoNEET inhibitor with antileukemic effect, which induces a concentration-dependent decrease in cell viability of REH and REH/Ara-C, and impaires the migratory ability of leukemic cells irrespective of the chemoattractant used.[2]
Cell Research Cell lines REH, REH/Ara-C and ALL (SUP-B15, TOM-1, JM1, NALM-1, NALM-6, BV-173) cell lines
Concentrations 10, 20, 30, 40, 50, 60, 70, 80, 100 μM
Incubation Time 72 h
Method

ALL cell lines are plated at 5×104cells/well in a 96-well plate. Following treatment with NL-1, viable cells are quantitated using a Cell Counting Kit-8.
In Vivo
In vivo

NL-1 shows antileukemic activity in an in vivo mouse ALL model.[2]
Animal Research Animal Models Female NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (6-8 month old) injected with TOM-1 ALL cells
Dosages 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 335.46 Formula

C18H25NO3S
CAS No. 188532-26-5 SDF --
Smiles CC(C)(C)C1=CC(=CC(=C1O)C(C)(C)C)CC2C(=O)NC(=O)S2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.72 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 8 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy NL-1 | NL-1 supplier | purchase NL-1 | NL-1 cost | NL-1 manufacturer | order NL-1 | NL-1 distributor