Home Microbiology Antiviral inhibitor NGI-1 For research use only.

NGI-1

Synonyms: ML414

NGI-1 is an aminobenzamide-sulfonamide compound that targets both oligosaccharyltransferase (OST) isoforms and therefore may exhibit antiviral activity against flaviviruses.

NGI-1 Chemical Structure

NGI-1 Chemical Structure

CAS No. 790702-57-7

Purity & Quality Control

Batch: S875001 DMSO]79 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.9%
99.9

NGI-1 Related Products

Biological Activity

Description NGI-1 is an aminobenzamide-sulfonamide compound that targets both oligosaccharyltransferase (OST) isoforms and therefore may exhibit antiviral activity against flaviviruses.
Targets
oligosaccharyltransferase (OST) [1]
In vitro
In vitro

NGI-1 is a reversible catalytic subunit inhibitor of the oligosaccharyltransferase (OST) that has higher specificity for STT3B compared to STT3A (STT: OST catalytic subunit). In non-small cell lung cancer cells NGI-1 blocks cell surface localization and signaling of the EGFR glycoprotein, but selectively arrests proliferation in only those cell lines that are dependent on EGFR (or FGFR) for survival. In these cell lines OST inhibition causes cell cycle arrest accompanied by induction of p21, autofluorescence, and changes in cell morphology; all hallmarks of senescence[1]. NGI-1 exhibits broad antiviral activity against multiple flaviviruses. It blocks viral RNA replication independent of inhibition of the catalytic activity of the OST complex[2].
Cell Research Cell lines PC9 and A549 cells
Concentrations 10 μM
Incubation Time 24 h
Method

PC9 and A549 cells were cultured in 6-well plates in serum-containing medium and treated with or without 10 μM NGI-1 for 24 hours.

Chemical Information & Solubility

Molecular Weight 394.51 Formula

C17H22N4O3S2
CAS No. 790702-57-7 SDF --
Smiles CC1=CN=C(S1)NC(=O)C2=C(C=CC(=C2)S(=O)(=O)N(C)C)N3CCCC3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 79 mg/mL ( (200.24 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy NGI-1 | NGI-1 supplier | purchase NGI-1 | NGI-1 cost | NGI-1 manufacturer | order NGI-1 | NGI-1 distributor