Home Microbiology Antiviral inhibitor GS-441524 For research use only.

GS-441524

GS-441524, a molecular precursor to a pharmacologically active nucleoside triphosphate molecule, is a potent inhibitor of feline infectious peritonitis virus (FIPV) with EC50 of 0.78 μM.

GS-441524 Chemical Structure

GS-441524 Chemical Structure

CAS No. 1191237-69-0

Purity & Quality Control

GS-441524 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HuH7 Function assay 10 uM 24 hrs Cmax in human HuH7 cells assessed as triphosphate concentration per million cells at 10 uM incubated for 24 hrs measured after washout by LC-MS analysis, Cmax=0.0000176μM 22446091
Vero Antiviral assay 24 hrs Antiviral activity against Parainfluenza 3 C 243 infected in african green monkey Vero cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=1.71μM 22446091
Vero Antiviral assay 24 hrs Antiviral activity against SARS coronavirus Toronto-2 infected in african green monkey Vero cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=2.24μM 22446091
HuH7 Antiviral assay 24 hrs Antiviral activity against Hepatitis C virus genotype 1b infected in human HuH7 cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=4.1μM 22446091
Vero E6 Antiviral assay 24 hrs Antiviral activity against Dengue virus type 2 New Guinea C infected in african green monkey Vero E6 cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=9.46μM 22446091
HeLa Antiviral assay 24 hrs Antiviral activity against Yellow fever virus 17D infected in human HeLa cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=11μM 22446091
MDCK Antiviral assay 24 hrs Antiviral activity against Influenza A virus H3N2 infected in MDCK cells assessed as inhibition of virus induced cytopathic effect after 24 hrs by MTS assay, EC50=27.9μM 22446091
Hep2 Antiviral assay 4 days Antiviral activity against Respiratory syncytial virus A2 infected in human Hep2 cells assessed as reduction of virus-induced cytopathic effect after 4 days by Cell-Titer Glo assay, EC50=0.53μM 28124907
HMVEC Antiviral assay 3 to 4 days Antiviral activity against GFP-fused Ebolavirus infected in TERT-immortalized HMVEC assessed as reduction in viral replication preincubated with cells followed by viral infection measured after 3 to 4 days by fluorescence assay, EC50=0.78μM 28124907
HuH7 Antiviral assay 3 days Antiviral activity against Hepatitis C virus genotype 1b infected in human HuH7 cells preincubated with cells for 3 days followed by viral infection by luciferase assay, EC50=4.1μM 28124907
HuH7 Antiviral assay 3 days Antiviral activity against Ebolavirus infected in human HuH7 cells after 3 days by end point dilution assay, EC50=1.5μM 28792763
Click to View More Cell Line Experimental Data

Biological Activity

Description GS-441524, a molecular precursor to a pharmacologically active nucleoside triphosphate molecule, is a potent inhibitor of feline infectious peritonitis virus (FIPV) with EC50 of 0.78 μM.
Targets
FIPV [1]
(Cell-free assay)
0.78 μM(EC50)
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05996744 Terminated
COVID-19
Gilead Sciences
 December 26 2023 Phase 2|Phase 3
NCT05715528 Completed
COVID-19
Gilead Sciences
 February 8 2023 Phase 3
NCT05603143 Terminated
COVID-19
Gilead Sciences
 November 5 2022 Phase 3
NCT04582266 Completed
COVID-19
National Institute of Allergy and Infectious Diseases (NIAID)|Gilead Sciences
 March 31 2021 --
NCT04859244 Completed
COVID-19
Copycat Sciences LLC
 January 1 2021 Phase 1
NCT04539262 Completed
COVID-19
Gilead Sciences
 September 14 2020 Phase 1|Phase 2

Chemical Information & Solubility

Molecular Weight 291.26 Formula

C12H13N5O4
CAS No. 1191237-69-0 SDF --
Smiles C1=C2C(=NC=NN2C(=C1)C3(C(C(C(O3)CO)O)O)C#N)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 58 mg/mL ( (199.13 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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