Home Epigenetics Histone Methyltransferase inhibitor - PRMT inhibitor MS049 For research use only.

MS049

MS049 is a potent and selective inhibitor of PRMT4 and PRMT6 with IC50s of 34 nM and 43 nM, respectively.

MS049 Chemical Structure

MS049 Chemical Structure

CAS No. 2095432-59-8

Purity & Quality Control

Batch: S814701 DMSO]56 mg/mL]false]Water]56 mg/mL]false]Ethanol]50 mg/mL]false Purity: 99.48%
99.48

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Biological Activity

Description MS049 is a potent and selective inhibitor of PRMT4 and PRMT6 with IC50s of 34 nM and 43 nM, respectively.
Targets
PRMT4 [1]
(Cell-free assay)		 PRMT6 [1]
(Cell-free assay)
34 nM 43 nM
In vitro
In vitro MS049 does not inhibit any of the methyltransferases at 50 μM or demethylases at 10 μM and does not display appreciable binding to any of the methyllysine/methylarginine reader proteins using the DSF assay at 200 μM. MS049 demonstrated excellent selectivity over a broad range of epigenetic and nonepigenetic targets. In cellular assays, MS049 potently inhibits the methyltransferase activity of PRMT4 and PRMT6 and reduces levels of Med12me2a and H3R2me2a in HEK293 cells[1].
Cell Research Cell lines HEK293 cells
Concentrations 0, 1, 10, and 50 μM
Incubation Time 96 h
Method

HEK293 cells grown in DMEM supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 μg/mL) were seeded on 96-well plates at density 3000/well and treated with compounds at 0, 1, 10, and 50 μM concentrations for 96 h. The confluency was measured using IncuCyte ZOOM live cell imaging device and analyzed with IncuCyte ZOOM software based on phase contrast images.

Chemical Information & Solubility

Molecular Weight 321.29 Formula

C15H26Cl2N2O
CAS No. 2095432-59-8 SDF Download MS049 SDF
Smiles CNCCN1CCC(CC1)OCC2=CC=CC=C2.Cl.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 56 mg/mL ( (174.29 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 56 mg/mL

Ethanol : 50 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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