Home Epigenetics Histone Methyltransferase inhibitor - PRMT inhibitor HLCL-61 HCL For research use only.

HLCL-61 HCL

Synonyms: HLCL61

HLCL-61 hydrochloride is a potent and selective PRMT5 inhibitor for the treatment of acute myeloid leukemia.

HLCL-61 HCL Chemical Structure

HLCL-61 HCL Chemical Structure

CAS No. 1158279-20-9

Purity & Quality Control

Batch: S820901 DMSO]76 mg/mL]false]Ethanol]76 mg/mL]false]Water]Insoluble]false Purity: 99.96%
99.96

HLCL-61 HCL Related Products

Signaling Pathway

Histone Methyltransferase Signaling Pathways

Histone Methyltransferase Signaling Pathway

Biological Activity

Description HLCL-61 hydrochloride is a potent and selective PRMT5 inhibitor for the treatment of acute myeloid leukemia.
Targets
PRMT5 [1]
(In THP-1 cells)
16.74 μM
In vitro
In vitro HLCL-61 shows no inhibitory activity against the type I (PRMT1 and PRMT4) and type II (PRMT7) PRMT family members. HLCL-61 shows effective inhibition of symmetric arginine dimethylation (me2) of histones H3 and H4 in AML samples.Treatment of AML cell lines (MV4-11 and THP-1) and primary blasts with HLCL-61 also results in a decrease of cell viability. HLCL-61 is also effective in promoting apoptosis in MV4-11 and THP-1 cells after 48 h[1].
Cell Research Cell lines AML cell lines
Concentrations 25,50uM
Incubation Time 48h
Method CD11b expression is tested by flow cytometry on cells treated with DMSO or HLCL-61 (25 and 50 μM) for 48 h.

Chemical Information & Solubility

Molecular Weight 380.91 Formula

C23H24N2O.HCl
CAS No. 1158279-20-9 SDF Download HLCL-61 HCL SDF
Smiles CCN1C2=C(C=C(C=C2)CNCC3=CC=CC=C3OC)C4=CC=CC=C41.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 76 mg/mL ( (199.52 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 76 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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