Home Transmembrane Transporters TRP Channel activator - Antiviral inhibitor ML-SA1 For research use only.

ML-SA1

Synonyms: Mucolipin synthetic agonist 1

ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.

ML-SA1 Chemical Structure

ML-SA1 Chemical Structure

CAS No. 332382-54-4

Purity & Quality Control

Batch: S992601 DMSO]18 mg/mL]false]Ethanol]2 mg/mL]false]Water]Insoluble]false Purity: 99.84%
99.84

ML-SA1 Related Products

Biological Activity

Description

ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.
Targets
DENV2 [2]
(A549 cells cells)		 TRPML channel [1] ZIKV [2]
(A549 cells cells)
8.93 µM 52.99 µM
In vitro
In vitro

ML-SA1, as a potent inhibitors of DENV2 and ZIKV, can inhibit DENV2 and ZIKV by promoting lysosome acidification and protease activity to cause viral degradation.<sup><a class="sref" href="#s_ref">[2]</a></sup>
Cell Research Cell lines A549 cells, Huh7 cells
Concentrations 25 μM
Incubation Time 0-14 h
Method

A549 or Huh7 cells are seeded into 96-well plates at a density of 8 × 10<sup>3</sup> for 12 h, followed by incubation with various concentrations of eML-SA1. After 48 h of incubation, the cell are used for CCK-8 assay. A549 or Huh7 cells are seeded into 12-well plates at a density of 2 × 105 for 12 h and pretreated with 25 μM ML-SA1 for 24 h using DMSO as a control. Then, cells are incubated with 1 μM Lyso-Sensor Green DND-189 for 1 h at 37℃ and digested with 0.25% trypsin to generate a single cell suspension, which is used for Flow Cytometer to investigate the effect of ML-SA1 on lysosomal acidification.
In Vivo
In vivo

Administration of ML-SA1 before transient global ischemia ameliorated neurological dysfunction possibly through the promotion of autophagy and the inhibition of apoptosis.<sup><a class="sref" href="#s_ref">[3]</a></sup>
Animal Research Animal Models Transient global ischemia model mice (male; C57BL/6)
Dosages 0.3 μl/min
Administration i.c.

Chemical Information & Solubility

Molecular Weight 362.42 Formula

C22H22N2O3
CAS No. 332382-54-4 SDF --
Smiles CC1CC(C)(C)N(C(=O)CN2C(=O)C3=C(C=CC=C3)C2=O)C4=C1C=CC=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 18 mg/mL ( (49.66 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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