ML-SA1

Synonyms: Mucolipin synthetic agonist 1

ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.

ML-SA1 Chemical Structure

ML-SA1 Chemical Structure

CAS No. 332382-54-4

Purity & Quality Control

Batch: S992601 DMSO]18 mg/mL]false]Ethanol]2 mg/mL]false]Water]Insoluble]false Purity: 99.84%
99.84

ML-SA1 Related Products

Biological Activity

Description

ML-SA1 (Mucolipin synthetic agonist 1) is an activator of TRPML channels. ML-SA1 also inhibits Dengue virus 2 (DENV2) and Zika virus (ZIKV) by promoting lysosomal acidification and protease activity. The IC50 values of ML-SA1 against DENV2 RNA and ZIKV RNA are 8.93 μM and 52.99 μM, respectively. ML-SA1 induces autophagy. ML-SA1 can be used for the research of broad-spectrum antiviral.

Targets
DENV2 [2]
(A549 cells cells)
TRPML channel [1] ZIKV [2]
(A549 cells cells)
8.93 µM 52.99 µM
In vitro
In vitro

ML-SA1, as a potent inhibitors of DENV2 and ZIKV, can inhibit DENV2 and ZIKV by promoting lysosome acidification and protease activity to cause viral degradation.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Cell Research Cell lines A549 cells, Huh7 cells
Concentrations 25 μM
Incubation Time 0-14 h
Method

A549 or Huh7 cells are seeded into 96-well plates at a density of 8 × 10<sup>3</sup> for 12 h, followed by incubation with various concentrations of eML-SA1. After 48 h of incubation, the cell are used for CCK-8 assay. A549 or Huh7 cells are seeded into 12-well plates at a density of 2 × 105 for 12 h and pretreated with 25 μM ML-SA1 for 24 h using DMSO as a control. Then, cells are incubated with 1 μM Lyso-Sensor Green DND-189 for 1 h at 37℃ and digested with 0.25% trypsin to generate a single cell suspension, which is used for Flow Cytometer to investigate the effect of ML-SA1 on lysosomal acidification.

In Vivo
In vivo

Administration of ML-SA1 before transient global ischemia ameliorated neurological dysfunction possibly through the promotion of autophagy and the inhibition of apoptosis.<sup><a class="sref" href="#s_ref">[3]</a></sup>

Animal Research Animal Models Transient global ischemia model mice (male; C57BL/6)
Dosages 0.3 μl/min
Administration i.c.

Chemical Information & Solubility

Molecular Weight 362.42 Formula

C22H22N2O3

CAS No. 332382-54-4 SDF --
Smiles CC1CC(C)(C)N(C(=O)CN2C(=O)C3=C(C=CC=C3)C2=O)C4=C1C=CC=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 18 mg/mL ( (49.66 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 2 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy ML-SA1 | ML-SA1 supplier | purchase ML-SA1 | ML-SA1 cost | ML-SA1 manufacturer | order ML-SA1 | ML-SA1 distributor