Home Transmembrane Transporters TRP Channel inhibitor SKF96365 For research use only.

SKF96365

SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels.

SKF96365 Chemical Structure

SKF96365 Chemical Structure

CAS No. 130495-35-1

Purity & Quality Control

SKF96365 Related Products

Biological Activity

Description SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels.
Targets
TRPC [1]
In vitro
In vitro SKF-96365 exhibits protective activity against MPP+ injury in PC12 cells and significantly inhibits apoptotic cell death in PC12 cells after MPP+ administration. SKF-96365 does not exert effects on necrotic cell death induced by MPP+ insult in PC12 cells. Because of its non-selective activity, SKF-96365 has been demonstrated to have effects on multiple other Ca2+ channels: it not only blocks high-voltage-activated (HVA) Ca2+ channels at typically utilized test concentrations, but also potently inhibits low-voltage -activated (LVA) T-type Ca2+ channels in HEK293 cells. The exact effect of SKF-96365 on intracellular calcium homeostasis might dependent on cell types and experimental models used[2].
Cell Research Cell lines PC12 cells
Concentrations 1 µM, 10 µM or 50 µM 
Incubation Time 30 min
Method

To investigate whether SKF-96365 could protect PC12 cells from injury induced by MPP+ insult, cultured PC12 cells are pretreated with SKF-96365 in different concentrations (1 µM, 10 µM or 50 µM) 30 min before MPP+ addition. The cells viability is measured 24 h after MPP+ insult by using the cell proliferation reagent WST-1.
In Vivo
In vivo SKF-96365 treatment inhibited the calcium/calmodulin-dependent protein kinase IIγ (CaMKIIγ)/AKT signaling cascade in vitro and in vivo[4].
Animal Research Animal Models C57BL/6N mice
Dosages 0.02 μmol & 0.2 μmol/2 μl/mouse
Administration intrastriatally

Chemical Information & Solubility

Molecular Weight 402.91 Formula

C22H26N2O3.HCl
CAS No. 130495-35-1 SDF Download SKF96365 SDF
Smiles COC1=CC=C(C=C1)CCCOC(CN2C=CN=C2)C3=CC=C(C=C3)OC.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 80 mg/mL ( (198.55 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 80 mg/mL

Ethanol : 80 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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