Home DNA Damage/DNA Repair DNA alkylator chemical Methyl methanesulfonate For research use only.

Methyl methanesulfonate

Synonyms: MMS

Methyl methanesulfonate (MMS), a DNA alkylating agent, modifies both guanine (to 7-methylguanine) and adenine (to 3-methlyladenine) to cause base mispairing and replication blocks, respectively.

Methyl methanesulfonate Chemical Structure

Methyl methanesulfonate Chemical Structure

CAS No. 66-27-3

Purity & Quality Control

Methyl methanesulfonate Related Products

Biological Activity

Description Methyl methanesulfonate (MMS), a DNA alkylating agent, modifies both guanine (to 7-methylguanine) and adenine (to 3-methlyladenine) to cause base mispairing and replication blocks, respectively.
Targets
DNA alkylator [1]
In vitro
In vitro

Methyl methanesulfonate(MMS) is used for DNA damaging agent to induce mutagenesis. The repair of MMS-induced heat-labile damage requires the base excision repair protein XRCC1, and is independent of Homologous recombination in both S.cerevisiae and mammalian cells. [2]
Cell Research Cell lines EM9 cells , irs1SF cell lines, V3-3 cell lines, AA8 cells
Concentrations 1.5 μM
Incubation Time 0.5 h
Method

Flasks are inoculated with 4 × 106 cells for 4 h prior to a 24 h treatment with hydroxyurea or etoposide, or 28 h prior to a 0.5 h treatment with MMS or MNNG. After treatment, the cells are released from the flask by trypsinization and then used for pulsed-field gel electrophoresis.
In Vivo
In vivo

Methyl methanesulfonate(MMS) , as a genotoxic compound, has DNA damage potential in multiple organs such as the liver, kidneys and bone marrow in mice, confirmed by the in vivo comet assay.[3]
Animal Research Animal Models Seven-week-old male Crli:CD1(ICR) mice
Dosages 50mg/kg, 100mg/kg, 150 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05316220 Not yet recruiting
Ulcerative Colitis (UC)
AbbVie
 September 15 2024 Phase 3
NCT05806255 Not yet recruiting
Palliative Care
William Osler Health System|Humber River Hospital|The Ottawa Hospital|Bruyere Research Institute|University of Ottawa|Ottawa Hospital Research Institute|University of Toronto|Queen''s University|McMaster University
 July 1 2024 Not Applicable
NCT05703373 Not yet recruiting
Pregnancy Related
University of Pennsylvania
 June 2024 Not Applicable
NCT06254950 Recruiting
Ulcerative Colitis
Takeda
 March 29 2024 Phase 2

Chemical Information & Solubility

Molecular Weight 110.13 Formula

C2H6O3S
CAS No. 66-27-3 SDF --
Smiles CO[S](C)(=O)=O
Storage (From the date of receipt) 2 years -20°C liquid

In vitro
Batch:

DMSO : 22 mg/mL ( (199.76 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 22 mg/mL

Ethanol : 22 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Methyl methanesulfonate | Methyl methanesulfonate supplier | purchase Methyl methanesulfonate | Methyl methanesulfonate cost | Methyl methanesulfonate manufacturer | order Methyl methanesulfonate | Methyl methanesulfonate distributor