Methyl methanesulfonate

Catalog No.E0609 Batch:E060902

Technical Data

Formula	C₂H₆O₃S
Molecular Weight	110.13	CAS No.	66-27-3
Solubility (25°C)*	In vitro


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Biological Activity

Description

Methyl methanesulfonate (MMS), a DNA alkylating agent, modifies both guanine (to 7-methylguanine) and adenine (to 3-methlyladenine) to cause base mispairing and replication blocks, respectively.

Targets

DNA alkylator ^[1]

In vitro

Methyl methanesulfonate(MMS) is used for DNA damaging agent to induce mutagenesis. The repair of MMS-induced heat-labile damage requires the base excision repair protein XRCC1, and is independent of Homologous recombination in both S.cerevisiae and mammalian cells. ^[2]

In vivo

Methyl methanesulfonate(MMS) , as a genotoxic compound, has DNA damage potential in multiple organs such as the liver, kidneys and bone marrow in mice, confirmed by the in vivo comet assay.^[3]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines EM9 cells , irs1SF cell lines, V3-3 cell lines, AA8 cells Concentrations 1.5 μM Incubation Time 0.5 h Method Flasks are inoculated with 4 × 10⁶ cells for 4 h prior to a 24 h treatment with hydroxyurea or etoposide, or 28 h prior to a 0.5 h treatment with MMS or MNNG. After treatment, the cells are released from the flask by trypsinization and then used for pulsed-field gel electrophoresis.
Animal Study:^{^[3]}	Animal Models Seven-week-old male Crli:CD1(ICR) mice Dosages 50mg/kg, 100mg/kg, 150 mg/kg Administration i.p.

Cell Assay:^{^[2]}

Cell lines
EM9 cells , irs1SF cell lines, V3-3 cell lines, AA8 cells
Concentrations
1.5 μM
Incubation Time
0.5 h
Method
Flasks are inoculated with 4 × 10⁶ cells for 4 h prior to a 24 h treatment with hydroxyurea or etoposide, or 28 h prior to a 0.5 h treatment with MMS or MNNG. After treatment, the cells are released from the flask by trypsinization and then used for pulsed-field gel electrophoresis.

Animal Study:^{^[3]}

Animal Models
Seven-week-old male Crli:CD1(ICR) mice
Dosages
50mg/kg, 100mg/kg, 150 mg/kg
Administration
i.p.

References

Selleck's Methyl methanesulfonate has been cited by 3 publications

Olaparib combined with CDK12-IN-3 to promote genomic instability and cell death in ovarian cancer [ Int J Biol Sci, 2024, 20(11):4513-4531]	PubMed: 39247812
The ARID1A-METTL3-m6A axis ensures effective RNase H1-mediated resolution of R-loops and genome stability [ Cell Rep, 2024, 43(2):113779]	PubMed: 38358891
APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress [ J Transl Med, 2023, 21(1):183]	PubMed: 36894994

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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