Home Metabolism Phospholipase (e.g. PLA) activator m-3M3FBS For research use only.

m-3M3FBS

m-3M3FBS is a potent activator of phospholipase C (PLC) that stimulates a transient intracellular calcium concentration increase in neutrophils. m-3M3FBS induces apoptosis in monocytic leukemia cells.

m-3M3FBS Chemical Structure

m-3M3FBS Chemical Structure

CAS No. 200933-14-8

Purity & Quality Control

m-3M3FBS Related Products

Signaling Pathway

Phospholipase (e.g. PLA) Signaling Pathways

Phospholipase (e.g. PLA) Signaling Pathway

Biological Activity

Description m-3M3FBS is a potent activator of phospholipase C (PLC) that stimulates a transient intracellular calcium concentration increase in neutrophils. m-3M3FBS induces apoptosis in monocytic leukemia cells.
Targets
PLC [1]
In vitro
In vitro

M-3M3FBS induces a calcium increase in all cell lines (human neutrophils, HL60, differentiated HL60, U937, NIH 3T3, and PC12) with no cell-type specificity, and stimulates neutrophil activity by directly activating Phospholipase C (PLC), which leads to superoxide generation.[1]
Cell Research Cell lines human neutrophils, HL60, differentiated HL60, U937, NIH 3T3, and PC12
Concentrations 2.5, 5, 10, 20, and 25 µM
Incubation Time --
Method

Freshly prepared human neutrophils were incubated in serum-free RPMI 1640 medium with 3 µM fura-2/acetoxymethyl ester at 37°C for 30 min with continuous stirring. After washing with serum-free RPMI 1640 medium, the cells were suspended in serum_x005ffree RPMI containing 250 µM of sulfinpyrazone to prevent dye leakage. Approximately 2×106 cells were suspended in Ca2+-free Locke’s solution for each measurement. Changes in the fluorescence ratio were measured at an emission wavelength of 500 nm for dual excitation wavelengths at 340 nm and 380 nm. The calibration of the fluorescence ratio versus [Ca2+]i was performed.

Chemical Information & Solubility

Molecular Weight 343.36 Formula

C16H16F3NO2S
CAS No. 200933-14-8 SDF --
Smiles CC1=CC(=C(C(=C1)C)S(=O)(=O)NC2=CC=CC(=C2)C(F)(F)F)C
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 69 mg/mL ( (200.95 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 69 mg/mL

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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