m-3M3FBS

Catalog No.S0982 Batch:S098202

Technical Data

Formula	C₁₆H₁₆F₃NO₂S
Molecular Weight	343.36	CAS No.	200933-14-8
Solubility (25°C)*	In vitro	DMSO	69 mg/mL (200.95 mM)
		Ethanol	69 mg/mL (200.95 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

m-3M3FBS is a potent activator of phospholipase C (PLC) that stimulates a transient intracellular calcium concentration increase in neutrophils. m-3M3FBS induces apoptosis in monocytic leukemia cells.

Targets

PLC ^[1]

In vitro

M-3M3FBS induces a calcium increase in all cell lines (human neutrophils, HL60, differentiated HL60, U937, NIH 3T3, and PC12) with no cell-type specificity, and stimulates neutrophil activity by directly activating Phospholipase C (PLC), which leads to superoxide generation.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines human neutrophils, HL60, differentiated HL60, U937, NIH 3T3, and PC12 Concentrations 2.5, 5, 10, 20, and 25 µM Incubation Time -- Method Freshly prepared human neutrophils were incubated in serum-free RPMI 1640 medium with 3 µM fura-2/acetoxymethyl ester at 37°C for 30 min with continuous stirring. After washing with serum-free RPMI 1640 medium, the cells were suspended in serum_x005ffree RPMI containing 250 µM of sulfinpyrazone to prevent dye leakage. Approximately 2×10⁶ cells were suspended in Ca²⁺-free Locke’s solution for each measurement. Changes in the fluorescence ratio were measured at an emission wavelength of 500 nm for dual excitation wavelengths at 340 nm and 380 nm. The calibration of the fluorescence ratio versus [Ca²⁺]i was performed.

Cell Assay:^{^[1]}

Cell lines
human neutrophils, HL60, differentiated HL60, U937, NIH 3T3, and PC12
Concentrations
2.5, 5, 10, 20, and 25 µM
Incubation Time
--
Method
Freshly prepared human neutrophils were incubated in serum-free RPMI 1640 medium with 3 µM fura-2/acetoxymethyl ester at 37°C for 30 min with continuous stirring. After washing with serum-free RPMI 1640 medium, the cells were suspended in serum_x005ffree RPMI containing 250 µM of sulfinpyrazone to prevent dye leakage. Approximately 2×10⁶ cells were suspended in Ca²⁺-free Locke’s solution for each measurement. Changes in the fluorescence ratio were measured at an emission wavelength of 500 nm for dual excitation wavelengths at 340 nm and 380 nm. The calibration of the fluorescence ratio versus [Ca²⁺]i was performed.

References

Selleck's m-3M3FBS has been cited by 1 publication

Doxorubicin-Induced Platelet Activation and Clearance Relieved by Salvianolic Acid Compound: Novel Mechanism and Potential Therapy for Chemotherapy-Associated Thrombosis and Thrombocytopenia [ Pharmaceuticals (Basel), 2022, 15(12)1444]	PubMed: 36558895

Doxorubicin-Induced Platelet Activation and Clearance Relieved by Salvianolic Acid Compound: Novel Mechanism and Potential Therapy for Chemotherapy-Associated Thrombosis and Thrombocytopenia [ Pharmaceuticals (Basel), 2022, 15(12)1444]

PubMed: 36558895

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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