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Kaempferide

Synonyms: 4'-Methylkaempferol, 4'-O-Methylkaempferol, Kaempferol 4'-methyl ether

Kaempferide (4'-Methylkaempferol, 4'-O-Methylkaempferol, Kaempferol 4'-methyl ether), a natural compound derived from the roots of kaempferia galanga, has a variety of effects including anti-carcinogenic, anti-inflammatory, anti-oxidant, anti-bacterial and anti-viral properties.

Kaempferide Chemical Structure

Kaempferide Chemical Structure

CAS No. 491-54-3

Purity & Quality Control

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Signaling Pathway

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EGFR Signaling Pathway

Biological Activity

Description Kaempferide (4'-Methylkaempferol, 4'-O-Methylkaempferol, Kaempferol 4'-methyl ether), a natural compound derived from the roots of kaempferia galanga, has a variety of effects including anti-carcinogenic, anti-inflammatory, anti-oxidant, anti-bacterial and anti-viral properties.
In vitro
In vitro Kaempferide is able to induce apoptosis in the "side population" (SP, a subpopulation enriched with CSC in various cancers) cells in myeloma. It reverses the activity of drug efflux transporter[2]. Kaempferide is non-toxic to normal fibroblasts while inducing morphological changes, membrane flip-flop and nuclear membrane damage, characteristic of apoptosis in HeLa cells. It is highly cytotoxic to cervical cancer cells. Kaempferide-induced cytotoxicity is independent of cell cycle arrest[3].
Cell Research Cell lines KMS-11 cells
Concentrations 4-64 μM
Incubation Time 24 h
Method

Unfractionated cells are seeded at 20000 cells per well in a 96 well plate. After 24h of incubation with various concentration of KFD, PAR, and DMSO (vehicle control), MTS solution is added and absorbance at 490 nm is determined.
In Vivo
In vivo Kaempferide (Kae) remarkably improves cardiac function, alleviates myocardial injury via a decrease in myocardial enzyme levels, and attenuates myocardial infarct size in a dose-dependent manner. It attenuates I/R-induced myocardial injury through inhibition of the Nrf2 and cleaved caspase-3 signaling pathways via a PI3K/Akt/GSK 3β-dependent mechanism. Preconditioning treatment with Kae significantly decreases serum TNF-α, IL-6, C-reactive protein (CRP), MDA, and ROS levels, while increases serum levels of SOD. Nuclear factor erythroid 2-related factor 2 (Nrf2) and cleaved caspase-3 expression levels are downregulated, while phospho-Akt (p-Akt) and phospho-glycogen synthase kinase-3β (p-GSK-3β) expression levels are upregulated[1]. Kaempferide is non-toxic as assessed by acute and chronic toxicity studies in Swiss albino mice in vivo[3].
Animal Research Animal Models Ischemia/Reperfusion (I/R) Rat Model (Adult Sprague Dawley (SD) rats)
Dosages 0.1 mg/kg, 0.3 mg/kg, 1 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01199250 Not yet recruiting
Lynch Syndrome|Recurrent Uterine Corpus Carcinoma|Stage I Uterine Corpus Cancer|Stage II Uterine Corpus Cancer|Stage III Uterine Corpus Cancer|Stage IV Uterine Corpus Cancer
Gynecologic Oncology Group|National Cancer Institute (NCI)|GOG Foundation
 January 2100 --
NCT04662164 Withdrawn
Type 2 Diabetes Patients
Shanghai HEP Pharmaceutical Co. Ltd.
 December 2025 Phase 1|Phase 2
NCT06177132 Not yet recruiting
Vestibular Disorder
University Hospital Ghent|University Ghent
 November 2025 Not Applicable
NCT03277170 Not yet recruiting
Asthma; Status|Asthma in Children|Asthma Acute|Asthma Attack|Acute Asthma Exacerbation
Vanderbilt University Medical Center
 September 1 2025 Phase 2

Chemical Information & Solubility

Molecular Weight 300.26 Formula

C16H12O6
CAS No. 491-54-3 SDF --
Smiles COC1=CC=C(C=C1)C2=C(C(=O)C3=C(C=C(C=C3O2)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 60 mg/mL ( (199.82 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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